rfaA
168
modulator of lipid biosynthesis
locus
BSU_24330
Molecular weight
14.55 kDa
pI
4.41
function
control of fatty acid biosynthesis
product
modulator of lipid biosynthesis
essential
quasi
synonyms
yqhY, yqhY
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1302 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,529,926 → 2,530,333
Phenotypes of a mutant
the mutant readily acquires suppressor mutants that result in reduced activity of the [wiki|ACCase] [pubmed|28579978]
the mutant tends to acquire suppressor mutations that result in improved growth [Pubmed|28189581]
enhanced colony wrinkling upon knockdown [pubmed|36069446]
the mutant forms lipophilic clusters [pubmed|28579978]
severe growth inhibition upon addition of subinhibitory concentrations of mirubactin C [pubmed|36312962]
The protein
Protein family
asp23 family (with [protein|2AD4F0AA218D4B8864E43A3E501A5B7EFC6256FE|rfaB], according to UniProt)
Structure
[AF|P54519]
Paralogous protein(s)
[protein|2AD4F0AA218D4B8864E43A3E501A5B7EFC6256FE|rfaB]
[wiki|Localization]
cell poles [pubmed|28579978]
Expression and Regulation
Operons
genes
[gene|ADD1CE87C1C5BA44FFF5499EA5E155C4C53A1C22|accB]-[gene|1DFFEB155028A5C6B4B344769EF7DAAC14983322|accC]-[gene|FACE7176B1A1F354CF27FB30E46A1140FAFB02F0|rfaA ]
description
[Pubmed|7592499]
Biological materials
Mutant
MGNA-C367 (yqhY::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2365 NBRP B. subtilis, Japan]
GP1468 (Δ''yqhY''::''erm''), available in [wiki|Jörg Stülke]'s lab [pubmed|28579978]
GP1765 (Δ''yqhY''::''cat''), available in [wiki|Jörg Stülke]'s lab [pubmed|28579978]
BKE24330 (Δ[gene|FACE7176B1A1F354CF27FB30E46A1140FAFB02F0|rfaA ]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE24330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCAATTCACCTCCGTAA, downstream forward: _UP4_TAAATGGCTTAACACGAAAC
BKK24330 (Δ[gene|FACE7176B1A1F354CF27FB30E46A1140FAFB02F0|rfaA ]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK24330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCAATTCACCTCCGTAA, downstream forward: _UP4_TAAATGGCTTAACACGAAAC
Expression vectors
GP1474 (chromosomal ''yqhY''-Strep fusion, ''aphA''3), purification from ''B. subtilis'', for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab
pGP1322 (N-terminal Strep-tag, purification from ''E. coli'', in [wiki|pGP172]), available in [wiki|Jörg Stülke]'s lab
pGP1325 (N-terminal His-tag, purification from ''E. coli'', in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab
pGP1496 (His-tag, purification from ''E. coli'', in pET28a+), available in [wiki|Jörg Stülke]'s lab
pGP1498 (N-terminal His-tag, TEV-site, purification from ''E. coli'', in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab
pGP3619 expression of [gene|FACE7176B1A1F354CF27FB30E46A1140FAFB02F0|rfaA ]-Strep by [wiki|pGP382] in B. subtilis suitable for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab
pGP3630 (N-terminal 6xHis-SUMO-tag, purification from E. coli, in pET-SUMO), available in [wiki|Jörg Stülke]'s lab
pGP3644 (N-terminal GST-Tag, purification from ''E. coli'', in [wiki|pGEX-6P-1]), available in [wiki|Jörg Stülke]'s lab
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
FLAG-tag construct
GP1481 (spc, based on [wiki|pGP1331]), available in [wiki|Jörg Stülke]'s lab
GFP fusion
GP1471 (spc, based on [wiki|pBP43]), available in [wiki|Jörg Stülke]'s lab [pubmed|28579978]
References
Reviews
Original Publications
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Time of last update: 2024-11-11 05:38:26
Author of last update: Jstuelk