licT

licT
168

transcriptional antiterminator of the bglP-bglH-yxiE operon and the bglS gene

Locus
BSU_39080
Molecular weight
32.22 kDa
Isoelectric point
5.94
Protein length
277 aaSequenceBlast
Gene length
834 bpSequenceBlast
Function
control of beta-glucan and beta-glucoside utilization
Product
transcriptional antiterminator (BglG family)
Essential
no
Synonyms
licT
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG3711 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
4,012,866 → 4,013,699
Phenotypes of a mutant
no expression of the bglP-bglH operon
The protein
Catalyzed reaction/ biological activity
binding to the mRNAs of ''bglS and the bglP-bglH'' operon, causes transcription antitermination (in presence of salicin and absence of glucose)
Protein family
PRD-containing transcription factors
Domains
N-terminal RNA binding domain PubMed
2 x PRD (PTS regulation domains) PubMed
2 PRD domains (aa 65-170, aa 171-277) (according to UniProt)
Structure
1H99 (PDB) (PRDs) PubMed
1L1C (PDB) (complex with RAT) PubMed
P39805 (AlphaFold)
Modification
phosphorylation at His-100 in PRD-1 by phosphorylated BglP, inhibits LicT antitermination activity
phosphorylation at His-207 and/or His-269 in PRD-2 by His-P-HPr, stimulates LicT antitermination activity
Paralogous protein(s)
SacT, GlcT, SacY
Localization
cytoplasm, even distribution in the absence of the inducer salicin, subpolar localization in the presence of salicin PubMed
Expression and Regulation
Operons
Genes
licT-bglS
Description
PubMed
Regulation
expressed in the stationary phase (temporal activation) PubMed
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Open in new tab

licT→bglS

2025-03-13 18:55:24

ghost

87

c53f5f9d65bee2f49b36b261b08f54a3681748a8

E04D349CE33F16260854D95835D7F11AFB892F8F

Genes
yxiP-licT-bglS
Description
PubMed
Open in new tab

yxiP→bglS

2025-03-10 19:54:21

Jstuelk

137

c71d4e27ae884ff063a0224636e47b3376f616d1

E9284413C9C634EA6CE5E83A3A68A4BC61C0C559

Biological materials
Mutant
GP427 (ΔlicT-bglS::erm), available in Jörg Stülke's lab
BKE39080 (ΔlicT::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGGCATGTCCCTCCAAAT,  downstream forward: _UP4_TAATGAGAGCGCTGACATTT
BKK39080 (ΔlicT::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGGCATGTCCCTCCAAAT,  downstream forward: _UP4_TAATGAGAGCGCTGACATTT
Expression vectors
for expression, purification of both PRDs in E. coli with N-terminal His-tag, in pWH844: pGP165,  available in Jörg Stülke's lab
for expression, purification of RNA-binding domain in E. coli with N-terminal His-tag, in pWH844: pGP315,  available in Jörg Stülke's lab
for expression, purification of RNA-binding domain in E. coli with N-terminal His-tag and thrombin cleavage site, in pGP570: pGP572,  available in Jörg Stülke's lab
FLAG-tag construct
GP1221 (spc, based on pGP1331), available in Jörg Stülke's lab
GFP fusion
GP1225, licT-gfp, spc, based on pGP1870), available in Jörg Stülke's lab PubMed
GP1229, licT-yfp, spc), available in Jörg Stülke's lab PubMed
Labs working on this gene/protein
Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France
Josef Deutscher, Microbiology and Molecular Genetics, INRA Paris-Grignon, France
References
Reviews
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Original Publications
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FAC682CCD66056A8CBB9AE5F4BF51C1AADEBBD2C

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Time of last update: 2025-03-12 15:20:42

Author of last update: Jstuelk