nasD
168
assimilatory nitrite reductase (subunit)
locus
BSU_03300
Molecular weight
88.24 kDa
pI
4.95
function
utilization of nitrite as nitrogen source
product
assimilatory nitrite reductase (subunit)
essential
no
ec
1.7.1.4
synonyms
nasD, nasBC, nirB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1251 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
355,764 → 358,181
The protein
Catalyzed reaction/ biological activity
2 H2O + 3 NADP+ + NH4+ --> 5 H+ + 3 NADPH + nitrite (according to UniProt)
2 H2O + 3 NAD+ + NH4+ --> 5 H+ + 3 NADH + nitrite (according to UniProt)
Protein family
nitrite and sulfite reductase 4Fe-4S domain family (with [protein|A4C9E4D1163B703A917BFCF97A5F575B19EDDD8D|cysI], according to UniProt)
[wiki|Cofactors]
Fe-S cluster [Pubmed|11289299]
FAD [Pubmed|11289299]
Structure
[PDB|3KLJ] (from Clostridium acetobutylicum, corresponds to aa 5 ... 399, 32% identity) [pubmed|20017214]
[AF|P42435]
Paralogous protein(s)
[protein|3F87227DC3371746B3823730B6A7024D348C151E|nasB]
Expression and Regulation
Operons
genes
[gene|3F87227DC3371746B3823730B6A7024D348C151E|nasB]-[gene|EC8BF9A3BA88FE2A78C6F78A888E0D1E25C47BC5|nasC]-[gene|FAAC0F819AEC8E055CCEE86D93C8DE5584DC4B23|nasD]-[gene|A8998F46F68155A5C8AE2A2FBB95CA5A1635BB2B|nasE]-[gene|159A6D0D45B5F0F055228357FD9A3BF8E5A999E5|nasF]
description
[Pubmed|7836289]
regulation
''[protein|search|nasD]'': expressed under anaerobic conditions ([protein|search|ResD]) [Pubmed|10972836]
regulatory mechanism
[protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD]: activation, [Pubmed|10972836], in [regulon|protein:1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD regulon]
[protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]: activation, [PubMed|8799114,9765565], in [regulon|protein:857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA regulon]
[protein|EC6697D5D945B7E5083AFED9218748763C443278|nsrR]: repression, [Pubmed|16885456], in [regulon|protein:EC6697D5D945B7E5083AFED9218748763C443278|nsrR regulon]
[protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]: repression, [pubmed|12354229], in [regulon|protein:F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|7836289], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
genes
[gene|FAAC0F819AEC8E055CCEE86D93C8DE5584DC4B23|nasD]-[gene|A8998F46F68155A5C8AE2A2FBB95CA5A1635BB2B|nasE]-[gene|159A6D0D45B5F0F055228357FD9A3BF8E5A999E5|nasF]
description
[Pubmed|9765565]
regulation
''[protein|search|nasD]'': expressed under anaerobic conditions ([protein|search|ResD]) [Pubmed|10972836]
Biological materials
Mutant
1A973 ( ''nasD''::''phleo''), [Pubmed|7868621], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A973&Search=1A973 BGSC]
BKE03300 (Δ[gene|FAAC0F819AEC8E055CCEE86D93C8DE5584DC4B23|nasD]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE03300 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAGATGATCCGCTCCTT, downstream forward: _UP4_TAATGACAAAAACTATCATT
BKK03300 (Δ[gene|FAAC0F819AEC8E055CCEE86D93C8DE5584DC4B23|nasD]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK03300 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAGATGATCCGCTCCTT, downstream forward: _UP4_TAATGACAAAAACTATCATT
References
Reviews
Original Publications
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Time of last update: 2025-01-13 14:28:12
Author of last update: Jstuelk