gatB
168
production of glutamyl-tRNA(Gln)
locus
BSU_06690
Molecular weight
53.24 kDa
pI
4.9
function
translation
product
glutamyl-tRNA(Gln) amidotransferase (subunit B)
essential
yes
ec
6.3.5.7
synonyms
gatB, yerN
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0064 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
730,509 → 731,939
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
ATP + H2O + L-glutamine + L-glutamyl-tRNAGln --> ADP + H+ + L-glutamate + L-glutaminyl-tRNAGln + phosphate (according to UniProt)
ATP + H2O + L-aspartyl-tRNAAsn + L-glutamine --> ADP + 2 H+ + L-asparaginyl-tRNAAsn + L-glutamate + phosphate (according to UniProt)
Protein family
GatB/GatE family (single member, according to UniProt)
Structure
[PDB|2DF4] ([protein|FA88576A405F8C92C83BE74985039DC02E1F6C94|gatA]-[protein|F9C088D2DB3E99FDF961DE1B78A9B81279F204C9|gatB]-[protein|4A6AB40A91B4821B99E2FECD1C4E523321B8BDEA|gatC] complex from ''Staphylococcus aureus'') [Pubmed|16809541]
[AF|O30509]
Expression and Regulation
Operons
genes
[gene|4A6AB40A91B4821B99E2FECD1C4E523321B8BDEA|gatC]-[gene|FA88576A405F8C92C83BE74985039DC02E1F6C94|gatA]-[gene|F9C088D2DB3E99FDF961DE1B78A9B81279F204C9|gatB]
description
[pubmed|22383849]
Biological materials
Mutant
MGNA-B447 (yerN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1446 NBRP B. subtilis, Japan]
References
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Time of last update: 2024-12-21 14:51:54
Author of last update: Jstuelk