skfB
168
thioether bond forming radical SAM enzyme, catalyzes the first step in the maturation of [protein|DDB0022F50999AC52809D651C9CC5A5FDC71302C|spore killing factor]
locus
BSU_01920
Molecular weight
45.59 kDa
pI
4.93
function
maturation of [protein|DDB0022F50999AC52809D651C9CC5A5FDC71302C|spore killing factor]
product
AdoMet radical sactisynthase
essential
no
synonyms
skfB, ybcP, ybcQ
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0535 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
214,175 → 215,407
The protein
Catalyzed reaction/ biological activity
generates a thioether bond in [protein|DDB0022F50999AC52809D651C9CC5A5FDC71302C|skfA], which links the cysteine residue Cys4 with the α-carbon of the methionine residue Met12 [Pubmed|23282011]
Protein family
[wiki|Radical SAM superfamily] (according to UniProt)
[wiki|Cofactors]
[4Fe-4S] and [2Fe-2S] clusters [Pubmed|30217813,23282011]
Structure
[PDB|6EFN] [Pubmed|30217813]
[AF|O31423]
Expression and Regulation
Operons
genes
[gene|DDB0022F50999AC52809D651C9CC5A5FDC71302C|skfA]-[gene|F981C605C652D1A4429579A5F24608CE7F570834|skfB]-[gene|4DFF9924E07D834F37580D73E88451C1671C5111|skfC]-[gene|5886364199844356446C08F08664B301A714E74A|skfE]-[gene|77D93CAEF46EBBB9BAF570714445E194D5F2884E|skfF]-[gene|527AB8EAAF0A1AAD4E5B714B35ED9F78BECC17EC|skfG]-[gene|F31B12F80AFC3580FD33811A1C22E74F75DC1A85|skfH]
description
[Pubmed|16816204]
regulation
expressed under conditions that trigger sporulation ([wiki|Spo0A]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647,15687200 PubMed]
regulatory mechanism
[protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]: activation, [PubMed|14651647,15687200], in [regulon|protein:2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A regulon]
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [PubMed|15687200,17720793], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]
[protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP]: activation, [Pubmed|16816204], in [regulon|protein:638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|16452424], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
Northern blotting during during phosphate limitation showed an intense 0.25 kb '[protein|search|skfA]'-specific transcript, and a weaker 6.5 kb '[protein|search|skfA]-[protein|search|skfB]-[protein|search|skfC]-[protein|search|skfE]-[protein|search|skfF]-[protein|search|skfG]-[protein|search|skfH]' transcript.
Biological materials
Mutant
MGNA-C513 (ybcQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2511 NBRP B. subtilis, Japan]
BKE01920 (Δ[gene|F981C605C652D1A4429579A5F24608CE7F570834|skfB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE01920 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATAAAATCCCCCTTAAG, downstream forward: _UP4_GAAGTCAAGGCAGGGATTCA
BKK01920 (Δ[gene|F981C605C652D1A4429579A5F24608CE7F570834|skfB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK01920 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATAAAATCCCCCTTAAG, downstream forward: _UP4_GAAGTCAAGGCAGGGATTCA
References
Reviews
Research papers
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Time of last update: 2024-12-21 13:43:49
Author of last update: Jstuelk