nadA
168
quinolinate synthetase
locus
BSU_27850
Molecular weight
41.33 kDa
pI
5.72
function
NAD biosynthesis
product
quinolinate synthetase
essential
no
ec
2.5.1.72
synonyms
nadA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0379 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,845,955 → 2,847,061
The protein
Catalyzed reaction/ biological activity
ATP + L-threonyl-[protein] --> ADP + H+ + O-phospho-L-threonyl-[protein] (according to UniProt)
Protein family
quinolinate synthase A family (single member, according to UniProt)
[wiki|Cofactors]
Fe-S cluster [Pubmed|18959769]
Structure
[PDB|4HHE] (from Pyrococcus furiosus, 34% identity) [pubmed|23999292]
[AF|Q9KWZ1]
Expression and Regulation
Operons
genes
[gene|D30D059185DFB828AB0FD07D600DBC3FD0973198|nadB]-[gene|1368734DCC28C618AA173B865784BDDAEA912623|nadC]-[gene|F92F17578F06EF2292D0923662933463D7C86498|nadA]
description
[Pubmed|8444804]
regulation
repressed in the presence of nicotinic acid ([protein|search|NadR]) [Pubmed|16199587]
regulatory mechanism
[protein|510DE075EA964D22C42DEB171509F6CDA600A402|nadR]: repression, [Pubmed|16199587], in [regulon|protein:510DE075EA964D22C42DEB171509F6CDA600A402|nadR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8444804], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
An [wiki|search|ncRNA] is predicted for '[protein|search|nadB]' [PubMed|20525796]
Biological materials
Mutant
BKE27850 (Δ[gene|F92F17578F06EF2292D0923662933463D7C86498|nadA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCATTCGATTGTTTGA, downstream forward: _UP4_TAGATCAGAAATATTTTCCC
BKK27850 (Δ[gene|F92F17578F06EF2292D0923662933463D7C86498|nadA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCATTCGATTGTTTGA, downstream forward: _UP4_TAGATCAGAAATATTTTCCC
labs
[wiki|Alessandra Albertini], University of Pavia, Italy [http://www-1.unipv.it/genbiom/albertini.html homepage]
References
Page visits: 3465
Time of last update: 2025-04-30 21:27:16
Author of last update: Melvin.boenninger