maeN
168
Na+/malate symporter
locus
BSU_31580
Molecular weight
47.66 kDa
pI
8.41
function
malate uptake
product
Na+/malate symporter
essential
no
synonyms
maeN, yufR
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG3493 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,244,770 → 3,246,116
The protein
Protein family
sodium:citrate (SCF) symporter family (together with [protein|FEC0EDE9663FE62B2A1A4FA5B16B41048DDD723B|cimH], according to UniProt)
Structure
[PDB|5X9R] (from Klebsiella pneumoniae, 34% identity) [pubmed|28566738]
[AF|O05256]
Paralogous protein(s)
[protein|FEC0EDE9663FE62B2A1A4FA5B16B41048DDD723B|cimH]
[wiki|Localization]
cell membrane (according to UniProt)
Expression and Regulation
Operons
genes
[gene|F8F62635AA87E4E3A255D6F8FC621EF40954B520|maeN]
description
[Pubmed|21815947]
regulation
induced by malate ([protein|search|MalR])
regulatory mechanism
[protein|689CF50691E77E5A40437EF1E6B5083AF7CFCE08|malR]: activation, in [regulon|protein:689CF50691E77E5A40437EF1E6B5083AF7CFCE08|malR regulon]
additional information
the mRNA is substantially stabilized upon depletion of [gene|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]
Biological materials
Mutant
MGNA-B556 (yufR::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1555 NBRP B. subtilis, Japan]
1A642 (''maeN''::Tn917, erm) (available from the [http://bgsc.org/ BGSC] or in [wiki|Jörg Stülke]'s lab)
1A642 ( ''maeN''::''erm''), [Pubmed|3015878], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A642&Search=1A642 BGSC]
GP1449 (''erm''), available in [wiki|Stülke] lab
BKE31580 (Δ[gene|F8F62635AA87E4E3A255D6F8FC621EF40954B520|maeN]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE31580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCATACACCCCCATGT, downstream forward: _UP4_TAAACAGAAAGAGTTCCGTT
BKK31580 (Δ[gene|F8F62635AA87E4E3A255D6F8FC621EF40954B520|maeN]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK31580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCATACACCCCCATGT, downstream forward: _UP4_TAAACAGAAAGAGTTCCGTT
Expression vectors
expression of native ''maeN'' in ''B. subtilis'': pGP1951 (in [wiki|pBQ200]), available in [wiki|Stülke] lab
References
Reviews
Original Publications
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Time of last update: 2024-12-21 08:59:11
Author of last update: Melvin.boenninger