rqcU
168
ribosome quality control (RQC) upstream factor, senses collisions between stalled and translating ribosomes, splits stalled ribosomes and targets the nascent peptide for degradation by the ribosome quality control pathway
locus
BSU_28580
Molecular weight
87.23 kDa
pI
5.79
function
initiation of ribosome-associated translation quality control
product
ribosome quality control (RQC) upstream factor
essential
no
synonyms
mutSB, yshD, mutS2
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1193 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,920,936 → 2,923,293
Phenotypes of a mutant
hypersensitive to antibiotics that target ribosomes [pubmed|37205477]
enhanced formation of [gene|F1969E4C7BAAF70BCBE570F58350C12A3E417539|rpsB] or [gene|144D952B05EBBFF41EC92DF906543EA00475FE69|rpsE] suppressor mutants after mitomycin treatment [pubmed|34339280]
The protein
Catalyzed reaction/ biological activity
ATPase activity: ATP + H2O --> ADP + H+ + phosphate [pubmed|38177497,37205477]
splits stalled ribosomes and targets the nascent peptide for degradation by the ribosome quality control pathway [pubmed|38177497,37205477]
Protein family
DNA mismatch repair mutS family (with [protein|E2A8B04CD729332F9E6A13195181983F751F2F25|mutS], according to UniProt)
[wiki|Domains]
Smr domain (aa 710-785) (according to UniProt)
Structure
[AF|P94545]
Expression and Regulation
Operons
genes
[gene|EAD44AA7D72510B70308FD0C9F159DBEDAEBBB74|zapA]-[gene|93726C423313C653C4F71CB107FA12F686959E41|yshB]-[gene|D3954F2BCF6CE66505E4F48FCB4A61EE8D24F6D3|polX]-[gene|F8E82D9AE514B31892FE4530532691274ADA682B|rqcU]-[gene|18CEF3F1EE2E95A009699DE370CBB44F025B7B40|yshE]
description
[Pubmed|22383849]
regulation
constitutively expressed [Pubmed|23701187]
Biological materials
Mutant
MGNA-B007 (yshD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1006 NBRP B. subtilis, Japan]
1A845 ( ''mutSB''::''cat''), [Pubmed|11254128], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A845&Search=1A845 BGSC]
1A845 ( ''mutSB''::''cat''), [Pubmed|11254128], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A845&Search=1A845 BGSC]
BKE28580 (Δ[gene|F8E82D9AE514B31892FE4530532691274ADA682B|rqcU]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE28580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGATTGTGTGAGCCTCCT, downstream forward: _UP4_CTAAAATAAAAGGGAGTATG
BKK28580 (Δ[gene|F8E82D9AE514B31892FE4530532691274ADA682B|rqcU]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK28580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGATTGTGTGAGCCTCCT, downstream forward: _UP4_CTAAAATAAAAGGGAGTATG
labs
[wiki|Alessandra Albertini], University of Pavia, Italy [http://www-1.unipv.it/genbiom/albertini.html homepage]
References
Reviews
Original Publications
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Time of last update: 2024-11-20 23:17:12
Author of last update: Jstuelk