rqcU

rqcU
168

ribosome quality control (RQC) upstream factor, senses collisions between stalled and translating ribosomes, splits stalled ribosomes and targets the nascent peptide for degradation by the ribosome quality control pathway

locus
BSU_28580
Molecular weight
87.23 kDa
pI
5.79
Protein length
Gene length
function
initiation of ribosome-associated translation quality control
product
ribosome quality control (RQC) upstream factor
essential
no
synonyms
mutSB, yshD, mutS2

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1193 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,920,936 → 2,923,293
Phenotypes of a mutant
hypersensitive to antibiotics that target ribosomes [pubmed|37205477]
enhanced formation of [gene|F1969E4C7BAAF70BCBE570F58350C12A3E417539|rpsB] or [gene|144D952B05EBBFF41EC92DF906543EA00475FE69|rpsE] suppressor mutants after mitomycin treatment [pubmed|34339280]
The protein
Catalyzed reaction/ biological activity
ATPase activity: ATP + H2O --> ADP + H+ + phosphate [pubmed|38177497,37205477]
splits stalled ribosomes and targets the nascent peptide for degradation by the ribosome quality control pathway [pubmed|38177497,37205477]
Protein family
DNA mismatch repair mutS family (with [protein|E2A8B04CD729332F9E6A13195181983F751F2F25|mutS], according to UniProt)
[wiki|Domains]
Smr domain (aa 710-785) (according to UniProt)
Structure
[AF|P94545]
Expression and Regulation
Operons
genes
[gene|EAD44AA7D72510B70308FD0C9F159DBEDAEBBB74|zapA]-[gene|93726C423313C653C4F71CB107FA12F686959E41|yshB]-[gene|D3954F2BCF6CE66505E4F48FCB4A61EE8D24F6D3|polX]-[gene|F8E82D9AE514B31892FE4530532691274ADA682B|rqcU]-[gene|18CEF3F1EE2E95A009699DE370CBB44F025B7B40|yshE]
description
[Pubmed|22383849]
regulation
constitutively expressed [Pubmed|23701187]
Open in new tab

[gene|EAD44AA7D72510B70308FD0C9F159DBEDAEBBB74|zapA]→[gene|18CEF3F1EE2E95A009699DE370CBB44F025B7B40|yshE]

2024-09-24 00:29:20

Jstuelk

106

a60f402276d016cd1954cecdf421cd68dd4fd667

C40CD769E454F6213CFF839AD02CA2F4B6A46C6B

Biological materials
Mutant
MGNA-B007 (yshD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1006 NBRP B. subtilis, Japan]
1A845 ( ''mutSB''::''cat''), [Pubmed|11254128], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A845&Search=1A845 BGSC]
1A845 ( ''mutSB''::''cat''), [Pubmed|11254128], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A845&Search=1A845 BGSC]
BKE28580 (Δ[gene|F8E82D9AE514B31892FE4530532691274ADA682B|rqcU]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE28580 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACGATTGTGTGAGCCTCCT,  downstream forward: _UP4_CTAAAATAAAAGGGAGTATG
BKK28580 (Δ[gene|F8E82D9AE514B31892FE4530532691274ADA682B|rqcU]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK28580 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACGATTGTGTGAGCCTCCT,  downstream forward: _UP4_CTAAAATAAAAGGGAGTATG
labs
[wiki|Alessandra Albertini], University of Pavia, Italy [http://www-1.unipv.it/genbiom/albertini.html homepage]
References
Reviews
22933559
Original Publications
11254128,27799325,30814990,34339280,35264791,37205477,38177497

F8E82D9AE514B31892FE4530532691274ADA682B

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Time of last update: 2024-10-06 02:24:46

Author of last update: Jstuelk