yqeU
168
ribosomal RNA small subunit methyltransferase E
locus
BSU_25440
Molecular weight
28.65 kDa
pI
5.29
function
rRNA maturation
product
ribosomal RNA small subunit methyltransferase E
essential
no
synonyms
yqeU, rsmE
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1385 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,623,032 → 2,623,802
The protein
Catalyzed reaction/ biological activity
S-adenosyl-L-methionine + uridine1498 in 16S rRNA --> H+ + N3-methyluridine1498 in 16S rRNA + S-adenosyl-L-homocysteine (according to UniProt)
Protein family
RNA methyltransferase rsmE family (single member, according to UniProt)
Structure
[PDB|1VHK] [pubmed|16021622]
[AF|P54461]
Expression and Regulation
Operons
genes
[gene|2E57DFB4162EF0CE759C1FC42C637E1B9E4B2F7E|hrcA]-[gene|ECAB685E9038DC03FA2CC659112BB07D30DE5C8C|grpE]-[gene|30E0AAABB803E577D0D9FBAEF9031319CABD3D89|dnaK]-[gene|D4C75B39B2DAECB9D85DAD66AAD4F923C9AEBEA7|dnaJ]-[gene|70D717806F5174447E01FF33DC8299CB025888CD|yqeT]-[gene|F8DC71B29D9C9BF4CF5124D0F01A71548306B9F9|yqeU]-[gene|6ADA5AF308D96218414EA92DFF4A57BAEAD0554B|yqeV]
description
[Pubmed|9023197]
regulation
induced by heat shock ([protein|search|HrcA]) [Pubmed|1339421]
regulatory mechanism
[protein|2E57DFB4162EF0CE759C1FC42C637E1B9E4B2F7E|hrcA]: repression, [Pubmed|1339421], in [regulon|protein:2E57DFB4162EF0CE759C1FC42C637E1B9E4B2F7E|hrcA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|1339421], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]
Biological materials
Mutant
MGNA-C497 (yqeU::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2495 NBRP B. subtilis, Japan]
BKE25440 (Δ[gene|F8DC71B29D9C9BF4CF5124D0F01A71548306B9F9|yqeU]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE25440 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACGCTGTGACACCTACT, downstream forward: _UP4_GAGTTATTAAGAGGTGATCA
BKK25440 (Δ[gene|F8DC71B29D9C9BF4CF5124D0F01A71548306B9F9|yqeU]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK25440 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACGCTGTGACACCTACT, downstream forward: _UP4_GAGTTATTAAGAGGTGATCA
References
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Time of last update: 2024-12-03 03:57:03
Author of last update: Melvin.boenninger