fur
168
transcription regulator of iron homoeostasis in response to iron availability
locus
BSU_23520
Molecular weight
17.26 kDa
pI
5.37
function
regulation of iron homoeostasis
product
transcriptional repressor [wiki|Fur family]
essential
no
synonyms
fur, yqkL
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0735 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,449,841 → 2,450,290
Phenotypes of a mutant
no growth with glucose and ammonium as single sources of carbon and nitrogen, respectively (due to [protein|CE542AE1A32CCD1ABB259E6159F7A37A8078E189|fsrA]-mediated repression of the ''[gene|44DF1B7E476AD58B3CCC39300FFE0132D0D32AD0|gltA]-[gene|AA07CC52B2DD48ACC9D3375E9D531CB1ACBE485A|gltB]'' operon) [Pubmed|22389480]
poor growth on lactate as single carbon source (due to overexpression of [protein|CE542AE1A32CCD1ABB259E6159F7A37A8078E189|fsrA]-mediated repression of the ''[gene|FEC83B66CEA311EA1650D61A215F82E9DF4E9F03|lutA]-[gene|57B9E37F6232189CD47C1B41FDCF43FCB8016AEB|lutB]-[gene|5EE69198882DF4E9AB8D9D7267F071EF88C2F16D|lutC]'' operon, can be suppressed by inactivation of ''[gene|CE542AE1A32CCD1ABB259E6159F7A37A8078E189|fsrA]'' or ''[gene|147AFEBEA546FDBEEB08E9A8D9C7BCDC9B83CC90|fbpB]'') [Pubmed|22427629]
severe growth inhibition upon addition of subinhibitory concentrations of mirubactin C [pubmed|36312962]
transcription profile of a ''[gene|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]'' mutant strain: [http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE27416 GEO] [Pubmed|22389480]
The protein
Catalyzed reaction/ biological activity
binds promoter regions of genes and operons of the [regulon|Fur regulon] to repress or stimulate transcription of the target genes [pubmed|39251624]
Protein family
[wiki|Fur family] (according to UniProt)
Structure
[PDB|4ETS] (from Campylobacter jejuni, 33% identity) [pubmed|22665794]
[AF|P54574]
Effectors of protein activity
DNA binding activity (repression) is triggered in the presence of Fe2+ [Pubmed|23057863]
in the absence of Fe2+, apo-[protein|E87DFC182D54EE96D4CA57607E734CDFB18E74EF|fpa] binds to [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur] and prevents its activity [pubmed|39251624]
Paralogous protein(s)
[protein|00BCAAE16576DC5426A652580C69A570FC7A1C2C|perR], [protein|A0CD8CCB54AE9F10DE3C876FB47B9877C303862D|zur]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]
description
[Pubmed|12029044]
regulation
repressed in the absence of hydrogen peroxide ([protein|search|PerR]) [Pubmed|12029044]
regulatory mechanism
[protein|00BCAAE16576DC5426A652580C69A570FC7A1C2C|perR]: repression, [Pubmed|12029044], in [regulon|protein:00BCAAE16576DC5426A652580C69A570FC7A1C2C|perR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|12029044], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
GP879 ([gene|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]::''mls'') and GP868 ([gene|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]::''mls'', [gene|00BCAAE16576DC5426A652580C69A570FC7A1C2C|perR]::''spc''), available in [wiki|Jörg Stülke]'s lab
GP3321 (Δ[gene|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]::''mls'' Δ[gene|E87DFC182D54EE96D4CA57607E734CDFB18E74EF|fpa]::''cat''), available in [wiki|Jörg Stülke]'s lab
HB6543 (aphA3), available in the [wiki|John Helmann]'s lab
MGNA-C426 (fur::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2424 NBRP B. subtilis, Japan]
1A910 ( ''fur''::''kan''), [Pubmed|12029044], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A910&Search=1A910 BGSC]
BKE23520 (Δ[gene|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCTTTCCCTCCTACGC, downstream forward: _UP4_TAGACGGTGCCGAGCGCGAA
BKK23520 (Δ[gene|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCTTTCCCTCCTACGC, downstream forward: _UP4_TAGACGGTGCCGAGCGCGAA
Expression vectors
pGP3589 (N-terminal 6xHis-SUMO-tag, purification from E. coli, in pET-SUMO), available in [wiki|Jörg Stülke]'s lab
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), pGP3869, pGP3871, pGP3873, pGP3875, available in [wiki|Jörg Stülke]'s lab
FLAG-tag construct
pGP3899 (C-terminal FLAG tag, based on [wiki|pGP1331])
lacZ fusion
pGP3592 (based on [wiki|pAC7]), available in [wiki|Jörg Stülke]'s lab
labs
[wiki|John Helmann], Cornell University, USA [http://www.micro.cornell.edu/research/labs/helmann-lab/index.cfm Homepage]
References
Reviews
Other original publications
The [wiki|Fur regulon]
Page visits: 7278
Time of last update: 2024-12-21 11:15:29
Author of last update: Jstuelk