brfA

brfA
168

release factor-dependent ribosome rescue factor

locus
BSU_23540
Molecular weight
7.95 kDa
pI
11.38
Protein length
Gene length
function
resolution of stalled translation complexes
product
ribosome rescue factor
essential
no
ec
null
synonyms
brfA, yqkK

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms

This gene is a member of the following regulons

Gene
Coordinates
2,451,148 → 2,451,363
Phenotypes of a mutant
a [gene|F7F780D11A6B11EB62CFA5202D1243A381AB40E1|brfA] [protein|D19519B5A776D6E8EC2BFE7A7D67469CA0A6459A|smpB] double mutant has a severe growth defect [pubmed|31776341]
The protein
Catalyzed reaction/ biological activity
binds to non-stop stalled ribosomes, recruits homologous [protein|12E06FD3A36D95AE82E3C65788FA98178039592F|RF2], but not [protein|59E6468FD891F5271F38543515867B0B4625768D|RF1], and induces its transition into an open active conformation [pubmed|31776341]
Structure
[PDB|6SZS] [pubmed|31776341]
[AF|P54573]
Biological materials
Mutant
MGNA-C457 (yqkK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2455 NBRP B. subtilis, Japan]
BKE23540 (Δ[gene|F7F780D11A6B11EB62CFA5202D1243A381AB40E1|brfA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23540 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTGATCACTCCCTTTTT,  downstream forward: _UP4_TAGCATTCATCTCTATTGTT
BKK23540 (Δ[gene|F7F780D11A6B11EB62CFA5202D1243A381AB40E1|brfA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23540 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTGATCACTCCCTTTTT,  downstream forward: _UP4_TAGCATTCATCTCTATTGTT
References
Reviews
33815344
Research papers
31776341,37496314,39026760

F7F780D11A6B11EB62CFA5202D1243A381AB40E1

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Time of last update: 2024-11-06 04:10:22

Author of last update: Jstuelk