brfA
168
release factor-dependent ribosome rescue factor
locus
BSU_23540
Molecular weight
7.95 kDa
pI
11.38
function
resolution of stalled translation complexes
product
ribosome rescue factor
essential
no
ec
null
synonyms
brfA, yqkK
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms
This gene is a member of the following regulons
Gene
Coordinates
2,451,148 → 2,451,363
Phenotypes of a mutant
a [gene|F7F780D11A6B11EB62CFA5202D1243A381AB40E1|brfA] [protein|D19519B5A776D6E8EC2BFE7A7D67469CA0A6459A|smpB] double mutant has a severe growth defect [pubmed|31776341]
The protein
Catalyzed reaction/ biological activity
binds to non-stop stalled ribosomes, recruits homologous [protein|12E06FD3A36D95AE82E3C65788FA98178039592F|RF2], but not [protein|59E6468FD891F5271F38543515867B0B4625768D|RF1], and induces its transition into an open active conformation [pubmed|31776341]
Structure
[PDB|6SZS] [pubmed|31776341]
[AF|P54573]
Biological materials
Mutant
MGNA-C457 (yqkK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2455 NBRP B. subtilis, Japan]
BKE23540 (Δ[gene|F7F780D11A6B11EB62CFA5202D1243A381AB40E1|brfA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTGATCACTCCCTTTTT, downstream forward: _UP4_TAGCATTCATCTCTATTGTT
BKK23540 (Δ[gene|F7F780D11A6B11EB62CFA5202D1243A381AB40E1|brfA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTGATCACTCCCTTTTT, downstream forward: _UP4_TAGCATTCATCTCTATTGTT
References
Reviews
Research papers
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Time of last update: 2024-12-01 17:50:56
Author of last update: Jstuelk