lysC
168
aspartokinase II (alpha and beta subunits)
locus
BSU_28470
Molecular weight
43.65 kDa
pI
4.64
function
biosynthesis of lysine
product
aspartokinase II (alpha and beta subunits)
essential
no
ec
2.7.2.4
synonyms
lysC, ask, aecA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0527 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,909,520 2,910,746
The protein
Catalyzed reaction/ biological activity
ATP + L-aspartate --> 4-phospho-L-aspartate + ADP (according to UniProt)
Protein family
aspartokinase family (with [protein|6D6718AC72C9D86EDE16B191EDAB3ABA488B689B|dapG] and [protein|548E92526BC00999031D6EDE43FE061CE0448AFC|thrD], according to UniProt)
[wiki|Domains]
two C-terminal [wiki|ACT domain]s (aa 264-337, and aa 343-408) (according to UniProt)
Structure
[PDB|2RE1] (from ''Neisseria meningitidis mc58'', 40% identity, 58% similarity)
[AF|P08495]
Effectors of protein activity
feedback inhibition by lysine [pubmed|15033471]
Paralogous protein(s)
[protein|6D6718AC72C9D86EDE16B191EDAB3ABA488B689B|dapG]
[wiki|Localization]
cytoplasm (according to UniProt)
Additional information
subject to Clp-dependent proteolysis upon glucose starvation [Pubmed|17981983], also degraded upon ammonium or amino acid starvation [Pubmed|2168395]
belongs to the 100 most abundant proteins [PubMed|15378759]
Expression and Regulation
Operons
genes
[gene|F7B947938FB40E54D89A719E9610C5A4AA400674|lysC]
description
[Pubmed|19636616]
regulation
expression activated by glucose (5.4 fold) [Pubmed|12850135]
regulatory mechanism
L-box: RNA switch, via a riboswitch, in [regulon|other_regulator:L-box|L-box]
Biological materials
Mutant
BKE28470 ([gene|F7B947938FB40E54D89A719E9610C5A4AA400674|lysC]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE28470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTATTACCACCCTTTAC, downstream forward: _UP4_TAATGACAATCAAAAAGGCG
BKK28470 ([gene|F7B947938FB40E54D89A719E9610C5A4AA400674|lysC]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK28470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTATTACCACCCTTTAC, downstream forward: _UP4_TAATGACAATCAAAAAGGCG
References
Reviews
Research papers
The [wiki|L-box] [wiki|riboswitch]
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Time of last update: 2025-03-01 21:11:17
Author of last update: Jstuelk