birA
168
trigger enzyme
locus
BSU_22440
Molecular weight
36.03 kDa
pI
6.77
function
regulation of biotin synthesis, addition of biotin to proteins
product
trigger enzyme
essential
yes
ec
6.3.4.15
synonyms
birA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1654 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,354,918 → 2,355,895
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
transcriptional repressor of the ''[gene|C40116089BAD3F9831F8C837DC4F2E045753ABDE|bioW]-[gene|68C86FED3575B864C576DFB73FE7B5BF83994D1F|bioA]-[gene|8B6A3E86A88928526D94FDA94B9C81ABF6E9ACFB|bioF]-[gene|D9A71DAF949FB6C9C22C8361D83C5A0741184977|bioD]-[gene|5880617EB95FC0D19219A868D6D0A3D1D0D9C532|bioB]-[gene|8FBC9A1AC108DC99E275D7A27C18C797D3CDB936|bioI]-[gene|089A32D08B5A7E552CBD9CB9745BD542863B4558|ytbQ]'' operon [Pubmed|8763940,8892842] '''
ATP + biotin + L-lysyl-[protein] --> AMP + diphosphate + H+ + N6-biotinyl-L-lysyl-[protein] (according to UniProt)
Protein family
biotin--protein ligase family (single member, according to UniProt)
[wiki|Domains]
[wiki|BPL/LPL catalytic domain] (aa 74-262) (according to UniProt)
Structure
[PDB|4HA8]
[AF|P0CI75]
Effectors of protein activity
biotinoyl-5'-AMP acts as co-repressor for DNA binding [pubmed|28466579]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|A828298EA2A002538876FBA6113648AAD9C4C317|ypjD]-[gene|5AB90032031653A913E15B26B3E1AE246E95AABF|dapB]-[gene|341F198B2BD4261B20C00181D084A7C5E33E39B3|mgsA]-[gene|60367F0396612C8A6491FD92A73B30BED7215506|bshB1]-[gene|187C78441816FC74B0FCCE34997E8AEC0A80B114|bshA]-[gene|4C4FA59FF5D100E941EF470C0C54E85681ADC74F|cca]-[gene|F761DBCE3429C99159F8F7CD8596FD9CF2D0589D|birA]
description
[Pubmed|20308541]
regulation
induced by diamide stess (thiol depletion) ([protein|search|Spx]) [Pubmed|23894131]
regulatory mechanism
[protein|2C6386E9A63F410558D168798D077DF91590F454|spx]: activation, [Pubmed|23894131], in [regulon|protein:2C6386E9A63F410558D168798D077DF91590F454|spx regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|23894131], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Expression vectors
pGP2690: expression in ''E. coli'', with N-terminal His-tag, in [wiki|pWH844], available in [wiki|Jörg Stülke]' lab
pGP2700: expression in ''B. subtilis'', with N-terminal Strep-tag, in [wiki|pGP380], available in [wiki|Jörg Stülke]' lab
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
lacZ fusion
pBP629 (in [wiki|pAC7]), available in [wiki|Fabian Commichau]'s lab
Antibody
available in [wiki|Jörg Stülke]'s lab
References
Reviews
Original Publications
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Time of last update: 2024-10-11 17:45:32
Author of last update: Melvin.boenninger