gcvT
168
aminomethyltransferase (glycine cleavage system protein T)
locus
BSU_24570
Molecular weight
39.62 kDa
pI
5.73
function
glycine utilization
product
aminomethyltransferase (glycine cleavage system protein T)
essential
no
ec
2.1.2.10
synonyms
gcvT, yqhI
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0404 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,548,245 → 2,549,333
The protein
Catalyzed reaction/ biological activity
(6S)-5,6,7,8-tetrahydrofolate + (R)-N6-(S8-aminomethyldihydrolipoyl)-L-lysyl-[protein] --> (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + (R)-N6-dihydrolipoyl-L-lysyl-[protein] + NH4+ (according to UniProt)
Protein family
gcvT family (single member, according to UniProt)
[wiki|Cofactors]
tetrahydrofolate
Structure
[PDB|1YX2]
[AF|P54378]
Expression and Regulation
Operons
genes
[gene|F6F449170276055E6D60D89E9E17A152A82CA166|gcvT]-[gene|DB0618EBA12013842ED3972165D0276F438D8EC1|gcvPA]-[gene|20175DF9FCF07C34A05C572B089C33D1E1FE6DF2|gcvPB]
description
[Pubmed|15472076]
regulation
induced by glycine [Pubmed|15472076]
the [wiki|Gly-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]
regulatory mechanism
Gly-box: RNA switch, [pubmed|31992591], in [regulon|other_regulator:Gly-box|Gly-box]
Biological materials
Mutant
MGNA-C428 (yqhI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2426 NBRP B. subtilis, Japan]
BKE24570 (Δ[gene|F6F449170276055E6D60D89E9E17A152A82CA166|gcvT]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE24570 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTAATTCCTCCCCTTTA, downstream forward: _UP4_TAATATTTTTTCTTGGAGAG
BKK24570 (Δ[gene|F6F449170276055E6D60D89E9E17A152A82CA166|gcvT]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK24570 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTAATTCCTCCCCTTTA, downstream forward: _UP4_TAATATTTTTTCTTGGAGAG
lacZ fusion
pGP431 (in [wiki|pAC7]), available in [wiki|Stülke] lab
References
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Time of last update: 2024-11-02 07:54:06
Author of last update: Jstuelk