yfmR
168
translation factor, housekeeping subfamily F ABC ATPase, prevents ribosome stalling during early elongation in the absence of [protein|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp]
locus
BSU_07370
Molecular weight
70.94 kDa
pI
5
function
prevents ribosome stalling in the absence of [protein|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp]
product
housekeeping subfamily F ABC ATPase
essential
no
synonyms
yfmR
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0488 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
809,557 → 811,446
Phenotypes of a mutant
an [gene|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp] [gene|F69F15198ACF8A8347C450EEC09F000EECEDEC41|yfmR] double mutant is not viable [pubmed|38943426,38349882]
The protein
Catalyzed reaction/ biological activity
resolves ribosome stalling on Asp-Pro motifs [pubmed|38943426]
Protein family
[wiki|ABC transporter superfamily] (according to UniProt)
[wiki|ABCF ATPase subfamily] [pubmed|30597160]
[wiki|Domains]
2 [wiki|ABC transporter domain]s (aa 4-255, aa 319-537) (according to UniProt)
Structure
[PDB|3J5S] (EttA from E. coli, 36% identity) [pubmed|24389465]
[PDB|4FIN] (EttA from E. coli, 36% identity) [pubmed|24389466]
[AF|O06476]
Paralogous protein(s)
[protein|1F56D3275386BB54A774BFA045C09E36D1268471|ydiF], [protein|2F79DB15D359A127C2D833CC0465E18E1725F539|ykpA], [protein|47B644F334A0BD501E77A198A61CE0F22BAB3E5E|yfmM]
Expression and Regulation
Operons
genes
[gene|F69F15198ACF8A8347C450EEC09F000EECEDEC41|yfmR]
description
[pubmed|22383849]
Biological materials
Mutant
MGNA-C234 (yfmR::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2232 NBRP B. subtilis, Japan]
BKE07370 (Δ[gene|F69F15198ACF8A8347C450EEC09F000EECEDEC41|yfmR]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE07370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGTTCCTATCAATCCTC, downstream forward: _UP4_TAAAAAGCGTGGCCGCAGCA
BKK07370 (Δ[gene|F69F15198ACF8A8347C450EEC09F000EECEDEC41|yfmR]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK07370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGTTCCTATCAATCCTC, downstream forward: _UP4_TAAAAAGCGTGGCCGCAGCA
References
Reviews
Original Publications
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Time of last update: 2024-12-21 09:48:50
Author of last update: Jstuelk