queE
168
7-carboxy-7-deazaguanine (CDG) synthase, required for the synthesis of the modified ribonucleotide queuosine
locus
BSU_13740
Molecular weight
26.97 kDa
pI
4.96
function
tRNA modification
product
7-carboxy-7-deazaguanine (CDG) synthase
essential
no
synonyms
queE, ykvL
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0602 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,440,542 → 1,441,273
The protein
Catalyzed reaction/ biological activity
radical-mediated ring rearrangement required to convert 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) into 7-carboxy-7-deazaguanine (CDG) [pubmed|29303575]
Protein family
[wiki|Radical SAM superfamily] (according to UniProt)
[wiki|Cofactors]
Fe-S cluster [pubmed|29292548]
S-adenosyl-L-methionine, Mg(2+) [Pubmed|23194065]
Structure
[PDB|5TGS] [pubmed|28045519]
[AF|O31677]
Expression and Regulation
Operons
genes
[gene|1925DFD1162E744E9175D07EC0FD72A75C79C6BF|queC]-[gene|BA6DD5C9AB9BFF9759E9ECC46CE3DF4EB7E67F52|queD]-[gene|F5189CAA5EF43FCF95E593E095F84B3BE486B347|queE]-[gene|5F96500CC46CC4DAECEBAD34EC50B10548E6D0F9|queF]
description
[Pubmed|14660578]
regulation
repressed in the presence of queuosine ([wiki|preQ1 riboswitch]) [Pubmed|19285444]
regulatory mechanism
preQ1 riboswitch: RNA switch, in the absence of queuosine [Pubmed|19285444], in [regulon|other_regulator:preQ1 riboswitch|preQ1 riboswitch]
Biological materials
Mutant
MGNA-A790 (ykvL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/790 NBRP B. subtilis, Japan]
BKE13740 (Δ[gene|F5189CAA5EF43FCF95E593E095F84B3BE486B347|queE]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE13740 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GAAAATTTCTAATACAGGAA, downstream forward: _UP4_GTATAATAGAAAGGAAGATG
BKK13740 (Δ[gene|F5189CAA5EF43FCF95E593E095F84B3BE486B347|queE]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK13740 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GAAAATTTCTAATACAGGAA, downstream forward: _UP4_GTATAATAGAAAGGAAGATG
References
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Time of last update: 2025-04-30 07:26:10
Author of last update: Jstuelk