ylaM
168
glutaminase, high affinity for glutamine
locus
BSU_14830
Molecular weight
33.86 kDa
pI
5.68
function
glutamine degradation
product
glutaminase
essential
no
ec
3.5.1.2
synonyms
ylaM
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2066 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,551,385 → 1,552,314
The protein
Catalyzed reaction/ biological activity
H2O + L-glutamine --> L-glutamate + NH4+ (according to UniProt)
Protein family
glutaminase family (with [protein|F31DD310F0D80ECC7C6D1E5DDB8EAF32565A3D51|ylaM], according to UniProt)
Structure
[PDB|3BRM] (the structure of [protein|1A415DC85354373EA4866733C3AE43F510BD3C56|glsA]) [Pubmed|18459799]
[AF|O07637]
Paralogous protein(s)
[protein|1A415DC85354373EA4866733C3AE43F510BD3C56|glsA]
Expression and Regulation
Operons
genes
[gene|5913ABC121A03644897BD365D7E507661CD3AF39|ylaK]-[gene|F31DD310F0D80ECC7C6D1E5DDB8EAF32565A3D51|ylaM]
description
[pubmed|22383849]
regulation
expressed during [wiki|sporulation] [pubmed|22383849]
Biological materials
Mutant
MGNA-A542 (ylaM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/542 NBRP B. subtilis, Japan]
BKE14830 (Δ[gene|F31DD310F0D80ECC7C6D1E5DDB8EAF32565A3D51|ylaM]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE14830 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCAAACACCCTCGTTCC, downstream forward: _UP4_TAATTTATGTCATATGCTTA
BKK14830 (Δ[gene|F31DD310F0D80ECC7C6D1E5DDB8EAF32565A3D51|ylaM]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK14830 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCAAACACCCTCGTTCC, downstream forward: _UP4_TAATTTATGTCATATGCTTA
References
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Time of last update: 2024-10-03 00:21:10
Author of last update: Jstuelk