yflG
168
methionine aminopeptidase
locus
BSU_07690
Molecular weight
27.06 kDa
pI
5.58
function
removal of N-terminal methionine from nascent proteins
product
methionine aminopeptidase
essential
no
synonyms
yflG, mapB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0024 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
839,735 → 840,484
The protein
Catalyzed reaction/ biological activity
Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides (according to UniProt)
Protein family
peptidase M24A family (with [protein|2F2455E189FC61A6B1EC6B66412FC662E44BD3F6|map], according to UniProt)
Structure
[PDB|1QXW] (from Staphylococcus aureus, 51% identity) [pubmed|14998322]
[AF|O34484]
Paralogous protein(s)
[protein|2F2455E189FC61A6B1EC6B66412FC662E44BD3F6|map]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|F2DE9BFE16D7B0DAE92DB58F9B1EDFBB7557ED16|yflG]-[gene|FB3A0E7A2428AA374C844C26464FAF8D40ADF142|yflH]-[gene|2BD3B1B122C902C01C88C97F0D5CD20962C9676B|yflI]
description
[Pubmed|22383849,16207374]
regulation
induced by stress ([protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]) [Pubmed|15805528]
regulatory mechanism
[protein|6D36D6360FE1CBFF5F64B2A6D1406405D6C6F7D5|nagR]: repression, (weak and not certain) [Pubmed|16207374], in [regulon|protein:6D36D6360FE1CBFF5F64B2A6D1406405D6C6F7D5|nagR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|16207374], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|15805528], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]
Biological materials
Mutant
MGNA-C255 (yflG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2253 NBRP B. subtilis, Japan]
BKE07690 (Δ[gene|F2DE9BFE16D7B0DAE92DB58F9B1EDFBB7557ED16|yflG]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE07690 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTGTCATTCCCGCTTTC, downstream forward: _UP4_TAAAACACATTCCGGGCTTC
BKK07690 (Δ[gene|F2DE9BFE16D7B0DAE92DB58F9B1EDFBB7557ED16|yflG]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK07690 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTGTCATTCCCGCTTTC, downstream forward: _UP4_TAAAACACATTCCGGGCTTC
References
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Time of last update: 2024-12-20 02:04:04
Author of last update: Melvin.boenninger