ywqL

ywqL
168

endonuclease V (endo V)

locus
BSU_36170
Molecular weight
26.83 kDa
pI
5.71
Protein length
Gene length
function
DNA excision repair
product
endonuclease V
essential
no
synonyms
ywqL

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1515 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,724,420 → 3,725,136
Phenotypes of a mutant
increased mutation rates [Pubmed|22056936]
increased sensitivity of sporulating cells to the genotoxic effects of nitrous acid[Pubmed|27698084]
increased mutation rates [pubmed|30726292]
The protein
Catalyzed reaction/ biological activity
Endonucleolytic cleavage at apurinic or apyrimidinic sites to products with a 5'-phosphate (according to UniProt)
in vitro endonuclease activity against double-stranded DNAs containing a single uracil (U), hypoxanthine (Hx), xanthine (X) or an AP site [pubmed|30726292]
catalyzes a single strand break at the second phosphodiester bond towards the 3'-end of the U and AP lesions [pubmed|30726292]
Protein family
endonuclease V family (single member, according to UniProt)
Structure
[PDB|3GA2]
[AF|P96724]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|2401A4E3E5E683472C51AB2F31BC4F54F672F32A|ywqH]-[gene|04376B3166B591132CE37D1DE85B0ECFEC6902EF|ywqI]-[gene|2F9C623DDF9F93EFD46F024FDA3AF2A92FC9BF7A|ywqJ]-[gene|7ED407717C216FCB2B281A3ECFAA2ED594F021AF|ywqK]-[gene|F2C6956D69FBE57933E46136436E8E80B63DF8BB|ywqL]
description
[Pubmed|22383849]
regulation
expressed throughout growth and staionary phase [Pubmed|22056936]
Open in new tab

[gene|2401A4E3E5E683472C51AB2F31BC4F54F672F32A|ywqH]→[gene|F2C6956D69FBE57933E46136436E8E80B63DF8BB|ywqL]

2024-10-05 04:32:12

ghost

101

7b0991ae05480ac928e975110362f8450bfcd9cc

255B293BB0BAC31EE5E24C2FA373EBECB756355C

genes
[gene|F2C6956D69FBE57933E46136436E8E80B63DF8BB|ywqL]
description
[Pubmed|9353933]
regulation
expressed throughout growth and staionary phase [Pubmed|22056936]
Open in new tab

[gene|F2C6956D69FBE57933E46136436E8E80B63DF8BB|ywqL]

2024-09-29 15:56:11

ghost

74

fe8cd19d2b7a8898dccefa244629758f01d3a223

4BE472B104D13FED9F0546902D8EAB920CE10112

Biological materials
Mutant
MGNA-A566 (ywqL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/566 NBRP B. subtilis, Japan]
BKE36170 (Δ[gene|F2C6956D69FBE57933E46136436E8E80B63DF8BB|ywqL]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE36170 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_ATCAAATACCTTCATAACGA,  downstream forward: _UP4_TAACACTTGGGGCTCTAAGT
BKK36170 (Δ[gene|F2C6956D69FBE57933E46136436E8E80B63DF8BB|ywqL]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK36170 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_ATCAAATACCTTCATAACGA,  downstream forward: _UP4_TAACACTTGGGGCTCTAAGT
References
Reviews
35210609,38551349
Research papers
9353933,22056936,22383849,27530626,27698084,30726292,35847079

F2C6956D69FBE57933E46136436E8E80B63DF8BB

Page visits: 3754

Time of last update: 2024-10-07 13:13:41

Author of last update: Jstuelk