manR
168
transcriptional activator of the [gene|575CEC5C5C0458DC86A74F24654AC6990CB1E732|manP]-[gene|E26C70893C5D677C816C814558CC42F90B920087|manA]-[gene|2DB0F763349D34FB1FCBBB4665416E129AD0AE6A|yjdF] operon
locus
BSU_12000
Molecular weight
75.13 kDa
pI
8.04
function
regulation of mannose utilization
product
transcriptional activator, PRD-type
essential
no
synonyms
manR, yjdC
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG3711 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,270,631 → 1,272,577
The protein
Catalyzed reaction/ biological activity
D-mannose + Nπ-phospho-L-histidyl-[protein] --> D-mannose 6-phosphate + L-histidyl-[protein] (according to UniProt)
Protein family
[wiki|PRD-containing transcription factors]
Structure
[AF|O31644]
Modification
phosphorylation by [protein|575CEC5C5C0458DC86A74F24654AC6990CB1E732|manP] inactivates [protein|F273002AF97D87BB025B4F014C328C5592EAD621|manR] (in the absence of mannose), phosphorylation by [gene|29B793660E4D30C0656248F3EF403FEF76FB9025|HPr] stimluates [protein|F273002AF97D87BB025B4F014C328C5592EAD621|manR] [Pubmed|20139185]
Expression and Regulation
Operons
genes
[gene|F273002AF97D87BB025B4F014C328C5592EAD621|manR]
description
[Pubmed|20139185]
regulation
induced by mannose ([protein|search|ManR]) [Pubmed|20139185]
regulatory mechanism
[protein|F273002AF97D87BB025B4F014C328C5592EAD621|manR]: activation, in the presence of mannose and absence of glucose [Pubmed|20139185], in [regulon|protein:F273002AF97D87BB025B4F014C328C5592EAD621|manR regulon]
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|22900538], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|20139185], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-A269 (yjdC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/269 NBRP B. subtilis, Japan]
BKE12000 (Δ[gene|F273002AF97D87BB025B4F014C328C5592EAD621|manR]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE12000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTGTTTTTCTATCCTTC, downstream forward: _UP4_TAAAAGCAGGGATTATTCCT
BKK12000 (Δ[gene|F273002AF97D87BB025B4F014C328C5592EAD621|manR]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK12000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTGTTTTTCTATCCTTC, downstream forward: _UP4_TAAAAGCAGGGATTATTCCT
References
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Time of last update: 2024-12-21 16:08:14
Author of last update: Melvin.boenninger