mccA
168
O-acetylserine-thiol-lyase
locus
BSU_27260
Molecular weight
32.86 kDa
pI
5.23
function
methionine-to-cysteine conversion
product
O-acetylserine-thiol-lyase
essential
no
synonyms
mccA, yrhA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0031 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,786,142 → 2,787,065
Phenotypes of a mutant
a [gene|F195C47849F4F6B4C56E3A386F5D8C84326B01C4|mccA] [gene|D1FC976597E5583E40A4ED7234FBCA743AB01354|cysK] double mutant is auxotrophic for cysteine [pubmed|17056751]
The protein
Catalyzed reaction/ biological activity
L-homocysteine + O-acetyl-L-serine --> acetate + H+ + L,L-cystathionine (according to UniProt)
Protein family
Cysteine synthase/cystathionine beta-synthase family (with [protein|D1FC976597E5583E40A4ED7234FBCA743AB01354|cysK] and [protein|6F1462EA074DEDE2FEF13561A6691A22BFAD10FE|ytkP], according to UniProt)
[wiki|Cofactors]
PLP (according to UniProt)
Structure
[PDB|4QL4] (from B. anthracis, 63% identity)
[AF|O05393]
Paralogous protein(s)
[protein|D1FC976597E5583E40A4ED7234FBCA743AB01354|cysK], [protein|6F1462EA074DEDE2FEF13561A6691A22BFAD10FE|ytkP]
[wiki|Localization]
cytoplasm (according to UniProt)
Expression and Regulation
Operons
genes
[gene|992A24CF9492965BF0E9EEFBB6F0A06871275068|yrrT]-[gene|5B21D1E73A65B13CE4DDA32ABC71EFA54E8D1D8F|mtnN]-[gene|F195C47849F4F6B4C56E3A386F5D8C84326B01C4|mccA]-[gene|314CB4923D15140E72B0ABAFE3E8BE9CA7310E10|mccB]-[gene|177EFFDCA6E3C3BB840EED13D9F2D71F99B833B6|yrhC]
description
[Pubmed|17056751]
regulation
repressed in the presence of cysteine ([protein|search|CymR]) [Pubmed|16513748]
regulatory mechanism
[protein|50930C56C27D22715620A350220E3C56ADB41020|cymR]: repression, [Pubmed|16513748], in [regulon|protein:50930C56C27D22715620A350220E3C56ADB41020|cymR regulon]
[protein|2C6386E9A63F410558D168798D077DF91590F454|spx]: activation, [Pubmed|12642660,16885442], in [regulon|protein:2C6386E9A63F410558D168798D077DF91590F454|spx regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|16513748], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-A849 (yrhA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/849 NBRP B. subtilis, Japan]
1A945 ( ''mccA''::''kan''), [Pubmed|17056751], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A945&Search=1A945 BGSC]
BKE27260 (Δ[gene|F195C47849F4F6B4C56E3A386F5D8C84326B01C4|mccA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATATGTCCTCCTCCTTA, downstream forward: _UP4_CAAATATACGAAGGAGGCAT
BKK27260 (Δ[gene|F195C47849F4F6B4C56E3A386F5D8C84326B01C4|mccA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATATGTCCTCCTCCTTA, downstream forward: _UP4_CAAATATACGAAGGAGGCAT
References
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Time of last update: 2024-12-21 15:50:16
Author of last update: Melvin.boenninger