secDF
168
preprotein translocase subunit (ATPase)
locus
BSU_27650
Molecular weight
81.47 kDa
pI
9.52
function
protein secretion
product
preprotein translocase subunit (ATPase)
essential
no
synonyms
secDF, secF, secD
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0342 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,826,900 → 2,829,113
Phenotypes of a mutant
reduced [wiki|protein secretion] [pubmed|32111210]
defective in [category|SW.4.1.4|Swarming] motility (prolonged period of immotility prior to [category|SW.4.1.4|Swarming]) [pubmed|35638827]
The protein
Protein family
N-terminal part: SecD/SecF family (single member, according to UniProt)
C-terminal part: SecD/SecF family (single member, according to UniProt)
Structure
[PDB|5XAN] (from Deinococcus radiodurans, 32% identity) [pubmed|28467902]
[AF|O32047]
[wiki|Localization]
membrane [Pubmed|18763711]
Expression and Regulation
Operons
genes
[gene|F21A75744FA0B25D5A251CB57E7A7DC6ABFF1DC7|comN]-[gene|F0E7B6E000A5B06C9023C76F21551E754C8C45BA|secDF]
description
[pubmed|22383849]
sigma factors
[protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]: sigma factor, [Pubmed|18179421], in [regulon|protein:081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM regulon]
Biological materials
Mutant
MGNA-B519 (yrvB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1518 NBRP B. subtilis, Japan]
SM-GN3 (''secDF-spc''), available in [wiki|Anne Galinier]'s and [wiki|Boris Görke]'s labs
BKE27650 (Δ[gene|F0E7B6E000A5B06C9023C76F21551E754C8C45BA|secDF]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27650 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATGTATATCCTCCCTT, downstream forward: _UP4_TAAAAAATATCAGGCTGTCC
BKK27650 (Δ[gene|F0E7B6E000A5B06C9023C76F21551E754C8C45BA|secDF]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27650 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATGTATATCCTCCCTT, downstream forward: _UP4_TAAAAAATATCAGGCTGTCC
References
Reviews
Original Publications
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Time of last update: 2024-12-21 09:39:04
Author of last update: Jstuelk