thiW
168
thiamine [wiki|ECF transporter] (ATP-binding protein)
locus
BSU_13220
Molecular weight
54.48 kDa
pI
5.71
function
thiamine uptake
product
thiamine [wiki|ECF transporter] (ATP-binding protein)
essential
no
synonyms
thiW, ykoD
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1123 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,388,809 → 1,390,452
The protein
Protein family
[wiki|ABC transporter superfamily] (according to UniProt)
[wiki|Domains]
2 [wiki|ABC transporter domain]s (aa 8-250, aa 295-523) (according to UniProt)
Structure
[PDB|4HUQ] (folate transporter from Lactobacillus brevis, 34% identity) [pubmed|23584589]
[AF|O34362]
[wiki|Localization]
membrane associated (via [protein|9AF27E4CE34B5C4036840A27D93B13213E3324DD|thiV]-[protein|236095EDE02EC9EA7768D9D30CED2345D3B7A1A9|thiX]) [Pubmed|16291685]
Expression and Regulation
Operons
genes
[gene|57A953B5D71F3FD8269EAF2366B8E2C7A8C79483|thiU]-[gene|9AF27E4CE34B5C4036840A27D93B13213E3324DD|thiV]-[gene|EF69891D9CF0CE57367B38FB3C69DDE1B689FCB7|thiW]-[gene|236095EDE02EC9EA7768D9D30CED2345D3B7A1A9|thiX]
description
[Pubmed|16291685]
regulation
repressed by thiamine ([protein|search|Thi-box]) [Pubmed|12376536]
regulatory mechanism
[protein|E195900D971AEE4A790D4579BE5E5A15B81010B8|Thi-box]: RNA switch, via [wiki|RNA switch], in [regulon|protein:E195900D971AEE4A790D4579BE5E5A15B81010B8|Thi-box regulon]
Biological materials
Mutant
MGNA-A755 (ykoD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/755 NBRP B. subtilis, Japan]
BKE13220 (Δ[gene|EF69891D9CF0CE57367B38FB3C69DDE1B689FCB7|thiW]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE13220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GAGCTGCTCAACCGTCAGAA, downstream forward: _UP4_ATGGCATTTCAAGAGGAGGT
BKK13220 (Δ[gene|EF69891D9CF0CE57367B38FB3C69DDE1B689FCB7|thiW]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK13220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GAGCTGCTCAACCGTCAGAA, downstream forward: _UP4_ATGGCATTTCAAGAGGAGGT
References
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Time of last update: 2024-09-27 19:10:41
Author of last update: Melvin.boenninger