yngG
168
3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) lyase
locus
BSU_18230
Molecular weight
32.49 kDa
pI
5.75
function
mother cell metabolism, leucine utilization
product
3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) lyase
essential
no
ec
4.1.3.4
synonyms
yngG
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0119 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,952,031 → 1,952,930
The protein
Catalyzed reaction/ biological activity
(S)-3-hydroxy-3-methylglutaryl-CoA --> acetyl-CoA + acetoacetate [Pubmed|19935659]
Protein family
HMG-CoA lyase family (single member, according to UniProt)
[wiki|Domains]
Pyruvate carboxyltransferase domain (aa 7-274)
Structure
[PDB|1YDO]
[AF|O34873]
Expression and Regulation
Operons
genes
[gene|BA28DFBAF88A4994B0541D1FF2187312FDE1FA15|yngJ]-[gene|5F73ACDEAFC7F1EB26D55195100C3283FC1F587C|yngI]-[gene|CAF58B4BAEB03353CF80A2862590888D07B48E2C|yngH]-[gene|1FBF1512C28BCE04E7D51247D94DC88C72FBFF07|yngHB]-[gene|EEEE69A98DFAA3327FBC04F1422E959D8B44AF20|yngG]-[gene|D5CCFDE9312909B7E5B98876794569357791C76B|yngF]-[gene|2192474E13EAF6D2CF97CB957864FC89ED0118E0|yngE]
description
[Pubmed|12662922]
regulation
expressed during sporulation in the mother cell ([protein|search|SigE]) [Pubmed|15699190,12662922]
sigma factors
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|15699190,12662922], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
Biological materials
Mutant
BKE18230 (Δ[gene|EEEE69A98DFAA3327FBC04F1422E959D8B44AF20|yngG]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE18230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATATGGCATGGTCTTCCTCC, downstream forward: _UP4_TGATAAAAGGGAGGACGCAT
BKK18230 (Δ[gene|EEEE69A98DFAA3327FBC04F1422E959D8B44AF20|yngG]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK18230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATATGGCATGGTCTTCCTCC, downstream forward: _UP4_TGATAAAAGGGAGGACGCAT
References
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Time of last update: 2024-12-30 19:12:31
Author of last update: Melvin.boenninger