tyrS

tyrS
168

tyrosyl-tRNA synthetase (major)

locus
BSU_29670
Molecular weight
47.57 kDa
pI
5.21
Protein length
Gene length
function
translation
product
tyrosyl-tRNA synthetase (major)
essential
quasi
ec
6.1.1.1
synonyms
tyrS

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0162 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,036,603 3,037,871
Phenotypes of a mutant
essential [Pubmed|12682299]
quasi-essential, the gene can be deleted, but the mutant acquires suppressors in [gene|2D4E3D6C3634C3F42A4517D5C1A8F4F48CAFF3A6|dtrR] to allow expression of [gene|1968743631A20739B5DA8B489CB1E7E42F3B93E3|tyrZ] [Pubmed|25733610]
a [gene|EEB0CF0AF0707AC1161F150708F7EDDB749B3C42|tyrS] [gene|1968743631A20739B5DA8B489CB1E7E42F3B93E3|tyrZ] double mutant is not viable [Pubmed|25733610]
Visit Sartorius.com Visit Sartorius.com
Advertisement
The protein
Catalyzed reaction/ biological activity
ATP + L-tyrosine + tRNATyr --> AMP + diphosphate + H+ + L-tyrosyl-tRNATyr (according to UniProt)
Protein family
[wiki|S4 RNA-binding domain] superfamily (according to http://www.ebi.ac.uk/interpro/entry/IPR036986 Interpro)
[wiki|Class-I aminoacyl-tRNA synthetase family] (according to UniProt)
[wiki|Domains]
[wiki|S4 RNA-binding domain] (aa 355-421) (according to UniProt)
Structure
[PDB|3TS1] (complex with tyrosyl adenylate intermediate, Geobacillus stearothermophilus)
[PDB|1JH3] (C-terminal domain, Geobacillus stearothermophilus)
[PDB|1N53] (the [regulon|T-box] antiterminator RNA) [Pubmed|12547201]
[AF|P22326]
Paralogous protein(s)
[protein|1968743631A20739B5DA8B489CB1E7E42F3B93E3|tyrZ]
Additional information
belongs to the 100 most abundant proteins [PubMed|15378759]
Expression and Regulation
Operons
genes
[gene|D50593AB6035B7DFEC76867B4692B069D91DEC10|ytzK]-[gene|EEB0CF0AF0707AC1161F150708F7EDDB749B3C42|tyrS]
description
[Pubmed|22383849]
regulation
the [wiki|T-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]
Open in new tab

[gene|D50593AB6035B7DFEC76867B4692B069D91DEC10|ytzK]→[gene|EEB0CF0AF0707AC1161F150708F7EDDB749B3C42|tyrS]

2024-05-20 21:55:14

Jstuelk

78

1eac0f916fed1a91bc71161f3775b2cc9c8c156a

752A20F00A4E21AABA296C0F1036E7E6A2A74E57

genes
[gene|6671E7D85E99D349679F0E9D825DC035D10FFD2E|acsA]-[gene|D50593AB6035B7DFEC76867B4692B069D91DEC10|ytzK]-[gene|EEB0CF0AF0707AC1161F150708F7EDDB749B3C42|tyrS]
description
[Pubmed|22383849]
regulation
repressed by glucose (4.5-fold) ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]) [Pubmed|7913927,12850135]
regulatory mechanism
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [pubmed|7913927], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
[protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: repression, [pubmed|12618455,18083814], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [pubmed|7913927], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Open in new tab

[gene|6671E7D85E99D349679F0E9D825DC035D10FFD2E|acsA]→[gene|EEB0CF0AF0707AC1161F150708F7EDDB749B3C42|tyrS]

2024-05-21 06:28:24

Jstuelk

87

64406ef718e7a3f606c729a8589083a4b11a12ee

AB9A54148FBC27339BE8203CF80C6070E696984B

genes
[gene|EEB0CF0AF0707AC1161F150708F7EDDB749B3C42|tyrS]
description
[Pubmed|1735721]
regulation
induced by tyrosine limitation ([regulon|T-box|]) [Pubmed|19258532]
the [wiki|T-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]
regulatory mechanism
T-box: RNA switch, in [regulon|other_regulator:T-box|T-box]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|1735721], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Open in new tab

[gene|EEB0CF0AF0707AC1161F150708F7EDDB749B3C42|tyrS]

2024-05-22 03:02:06

Jstuelk

57

963c0e314d542613c825b8b4b8aea900bed8d0a5

FC351833A926B6BA7EA33D2F1BAEC66C34BDD6D0

References
Reviews
19258532,10546897,11163353
Original Publications
9098057,12547201,11842119,10943892,9282752,8045882,8348614,1735721,8289305,20110252,21333656,15378759,25733610,25733611,29794222

EEB0CF0AF0707AC1161F150708F7EDDB749B3C42

Page visits: 2644

Time of last update: 2024-05-22 01:42:35

Author of last update: Jstuelk