murF
168
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine synthetase
locus
BSU_04570
Molecular weight
49.56 kDa
pI
5.34
function
peptidoglycan precursor biosynthesis
product
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6- diaminopimeloyl-D-alanyl-D-alanine synthetase
essential
yes
ec
6.3.2.10
synonyms
murF, ydbQ
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0770 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
509,384 → 510,757
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
ATP + D-alanyl-D-alanine + UDP-N-acetyl-α-D-muramoyl-L-alanyl-γ-D-glutamyl-L-lysine --> ADP + H+ + phosphate + UDP-N-acetyl-α-D-muramoyl-L-alanyl-γ-D-glutamyl-L-lysyl-D-alanyl-D-alanine (according to UniProt)
Protein family
[wiki|MurCDEF family] (according to UniProt)
Structure
[PDB|1GG4] (from ''Escherichia coli'', 32% identity, 50% similarity) [Pubmed|11090285]
[AF|P96613]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
forms banded patterns along the length of the cell, this pattern formation depends on [protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|mreB] [Pubmed|20233306]
Expression and Regulation
Operons
genes
[gene|A47DBF8546E0F726A0FE58C21D3F28150290DF7F|ddl]-[gene|EC9F1D4F1863E53A818A51B0E16B201A251F077E|murF]
description
[Pubmed|22383849]
regulation
expression is reduced in a [protein|D04629D798E458AD538809BB2B150B7FA81D2C7E|sigV] mutant [Pubmed|21926231]
the leader mRNA is processed upstream of [gene|A47DBF8546E0F726A0FE58C21D3F28150290DF7F|ddl] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|ricA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|ricF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT] complex [Pubmed|29794222]
sigma factors
[protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]: sigma factor, [Pubmed|18179421], in [regulon|protein:081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM regulon]
[protein|D04629D798E458AD538809BB2B150B7FA81D2C7E|sigV]: sigma factor, in [regulon|protein:D04629D798E458AD538809BB2B150B7FA81D2C7E|sigV regulon]
Biological materials
Mutant
MGNA-C105 (murF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2103 NBRP B. subtilis, Japan]
References
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Time of last update: 2024-12-22 04:05:40
Author of last update: Melvin.boenninger