nrgB
168
nitrogen-regulated PII protein
locus
BSU_36520
Molecular weight
12.68 kDa
pI
8.82
function
regulation of ammonium uptake
product
nitrogen-regulated PII protein
essential
no
synonyms
nrgB, glnK
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0347 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,758,016 → 3,758,366
The protein
Protein family
P(II) protein family (single member, according to UniProt)
Structure
[PDB|4R25] [Pubmed|25691471]
[http://pdb.org/pdb/search/structidSearch.do?structureId=4r25 4R25] [Pubmed|25691471]
[http://www.ebi.ac.uk/pdbe-srv/view/entry/2nuu/summary 2NUU] (the complex with [protein|796BD46066B1E6147048664FA78679B42FA9D628|nrgA], from ''E. coli'') [Pubmed|17220269]
[AF|Q07428]
[wiki|Localization]
cell membrane, via [protein|796BD46066B1E6147048664FA78679B42FA9D628|nrgA] [Pubmed|14600241]
Expression and Regulation
Operons
genes
[gene|796BD46066B1E6147048664FA78679B42FA9D628|nrgA]-[gene|EC7C2A0B5A9A30B2FAA0CF9EEB9830CE0E6F2219|nrgB]
description
[Pubmed|8282685]
regulation
induced by ammonium limitation
regulatory mechanism
[protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]: activation, [Pubmed|8799114], in [regulon|protein:857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8282685], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
GP4167 (Δ[gene|796BD46066B1E6147048664FA78679B42FA9D628|nrgA]-[gene|EC7C2A0B5A9A30B2FAA0CF9EEB9830CE0E6F2219|nrgB]::''spec''), available in [wiki|Jörg Stülke]'s lab
GP4168 (''ΔnrgB''::''phleo''), available in [wiki|Jörg Stülke]'s lab
GP259 (Δ[gene|EC7C2A0B5A9A30B2FAA0CF9EEB9830CE0E6F2219|nrgB]::cat), available in [wiki|Jörg Stülke]'s lab [Pubmed|14600241]
GP255 (Δ[gene|796BD46066B1E6147048664FA78679B42FA9D628|nrgA]-[gene|EC7C2A0B5A9A30B2FAA0CF9EEB9830CE0E6F2219|nrgB]::cat), available in [wiki|Jörg Stülke]'s lab [Pubmed|14600241]
GP4092 (Δ[gene|EC7C2A0B5A9A30B2FAA0CF9EEB9830CE0E6F2219|nrgB]::phleo), available in [wiki|Jörg Stülke]'s lab
BKE36520 (Δ[gene|EC7C2A0B5A9A30B2FAA0CF9EEB9830CE0E6F2219|nrgB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE36520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGACCGCTCATAGCGTCAC, downstream forward: _UP4_TAATATCGGTACGAGATTCG
BKK36520 (Δ[gene|EC7C2A0B5A9A30B2FAA0CF9EEB9830CE0E6F2219|nrgB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK36520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGACCGCTCATAGCGTCAC, downstream forward: _UP4_TAATATCGGTACGAGATTCG
Antibody
available in [wiki|Jörg Stülke]s lab [Pubmed|14600241]
labs
[wiki|Susan Fisher], Boston, USA [http://www.bumc.bu.edu/microbiology/research/susan-h-fisher-phd/ homepage]
References
Reviews
Original Publications
Page visits: 4195
Time of last update: 2025-01-15 12:28:25
Author of last update: Jstuelk