rpoB

rpoB
168

[wiki|RNA polymerase] beta subunit

locus
BSU_01070
Molecular weight
133.38 kDa
pI
4.73
Protein length
Gene length
function
[wiki|transcription]
product
[wiki|RNA polymerase] beta subunit
essential
yes
ec
2.7.7.6
synonyms
rpoB

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0085 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
121,919 → 125,500
Phenotypes of a mutant
essential [Pubmed|12682299]
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The protein
Catalyzed reaction/ biological activity
Nucleoside triphosphate + RNA(n) --> diphosphate + RNA(n + 1) (according to UniProt)
Protein family
[wiki|RNA polymerase] beta chain family (single member, according to UniProt)
Structure
[PDB|3IYD] ([wiki|RNA polymerase] from ''E. coli'', 59% identity) [Pubmed|19903881]
[PDB|3MLQ] (RNA polymerase interacting domain of ''Thermus thermophilus'' [protein|AD1F39823ABCE2A222259B879513752942C59590|mfd] with the ''Thermus aquaticus'' [protein|EC32328548BFB59D9026ACE351C946DBBC0932BC|rpoB] beta1 domain) [Pubmed|20702425]
[PDB|8EHQ] (the paused transcription complex of Mycobacterium tuberculosis [wiki|RNA polymerase] with B. subtilis NusG) [pubmed|36745813]
[PDB|8EOE] (the elongation complex of Mycobacterium tuberculosis [wiki|RNA polymerase] with B. subtilis NusG) [pubmed|36745813]
[AF|P37870]
Modification
phosphorylated on (Arg-312 OR Arg-313), Arg-539, (Arg-693 OR Arg-694), Arg-827, and Arg-1106 [Pubmed|22517742]
[wiki|Localization]
cytoplasm
Expression and Regulation
Operons
genes
[gene|EC32328548BFB59D9026ACE351C946DBBC0932BC|rpoB]-[gene|BC8B37355E6FADBA5F4636C3879E99003B0F3E84|rpoC]
description
[Pubmed|22383849]
regulation
[protein|CEE73284EFC0DBE8870CE0B474922DED79475A57|rel] dependent downregulation (Class I) during stringent response [Pubmed|11948165]
the leader mRNA is processed upstream of [gene|EC32328548BFB59D9026ACE351C946DBBC0932BC|rpoB] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|ricA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|ricF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT] complex [Pubmed|29794222]
regulatory mechanism
stringent response: negative regulation, in [regulon|other_regulator:stringent response|stringent response]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|7657605], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
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[gene|EC32328548BFB59D9026ACE351C946DBBC0932BC|rpoB]→[gene|BC8B37355E6FADBA5F4636C3879E99003B0F3E84|rpoC]

2024-07-13 14:24:29

Jstuelk

107

9ecfb91baa38d439fdb11db43c6bb767fe4b71dc

AA678F0994EDBB47CABD9A659CCE017143F2ECAA

Biological materials
two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Fabian Commichau]'s lab
FLAG-tag construct
GP2043 ''rpoB-3xFLAG ermC'' (based on [wiki|pGP1087]), available in [wiki|Jörg Stülke]'s lab
lacZ fusion
pGP2683 (cat) based on [wiki|pAC5], available in [wiki|Jörg Stülke]'s lab
pGP2682 (aphA3) based on [wiki|pAC7], available in [wiki|Jörg Stülke]'s lab
References
Reviews
22210308,24763425,28657884
Original Publications
36815589,19680289,17644585,24491578,7657605,12486038,10675340,7592585,20525796,17981983,18763711,19191541,21512256,21821766,22517742,24572018,22484477,20702425,20817769,26293966,26538577,19903881,26098117,28539362,29794222,33068413,33339820,33339827,34149677,34157109,34279225,34495706,35258334,36753546,36745813,36779708

EC32328548BFB59D9026ACE351C946DBBC0932BC

Page visits: 12525

Time of last update: 2024-07-15 05:32:48

Author of last update: Jstuelk