tcyP
168
cystine and diaminopimelate transporter
locus
BSU_09130
Molecular weight
48.82 kDa
pI
9.47
function
cystine and diaminopimelate uptake
product
cystine and diaminopimelate transporter
essential
no
synonyms
tcyP, yhcL
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1823 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
986,986 → 988,377
The protein
Catalyzed reaction/ biological activity
cystine-proton symporter [Pubmed|15262924]
transport of diaminopimelate [pubmed|29995990]
Protein family
[wiki|dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family] (according to UniProt)
Structure
[PDB|3KBC] (from ''Pyrococcus horikoshii'', 28% identity) [Pubmed|19924125]
[AF|P54596]
Kinetic information
K(m) = 0.6 myM cystine [Pubmed|15262924]
[wiki|Localization]
membrane associated [Pubmed|18763711]
Expression and Regulation
Operons
genes
[gene|EC287BBD7AB1EB44A33BF29144448F53D06AC6D9|tcyP]
regulation
repressed in the presence of sulfate or cysteine ([protein|search|CymR]) [Pubmed|16513748]
regulatory mechanism
[protein|50930C56C27D22715620A350220E3C56ADB41020|cymR]: repression, [Pubmed|16513748], in [regulon|protein:50930C56C27D22715620A350220E3C56ADB41020|cymR regulon]
Biological materials
Mutant
GP4481 (trpC2 Δ[gene|EC287BBD7AB1EB44A33BF29144448F53D06AC6D9|tcyP]::neo), available in [wiki|Jörg Stülke]'s lab
GP4678 (trpC2 Δ[gene|EC287BBD7AB1EB44A33BF29144448F53D06AC6D9|tcyP]::lox72), available in [wiki|Jörg Stülke]'s lab
GP4680 (trpC2 Δ[gene|EC287BBD7AB1EB44A33BF29144448F53D06AC6D9|tcyP]::ermC), available in [wiki|Jörg Stülke]'s lab
MGNA-A657 (yhcL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/657 NBRP B. subtilis, Japan]
1A949 ( ''tcyP''::''spec''), [Pubmed|15262924], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A949&Search=1A949 BGSC]
BKE09130 (Δ[gene|EC287BBD7AB1EB44A33BF29144448F53D06AC6D9|tcyP]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE09130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAGCAGGTAAACTCTCCC, downstream forward: _UP4_TAACATATGAAAACGTGTAA
BKK09130 (Δ[gene|EC287BBD7AB1EB44A33BF29144448F53D06AC6D9|tcyP]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK09130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAGCAGGTAAACTCTCCC, downstream forward: _UP4_TAACATATGAAAACGTGTAA
labs
[[Isabelle Martin-Verstraete]], Institute Pasteur, Paris, France
References
Page visits: 5550
Time of last update: 2025-04-18 03:23:10
Author of last update: Robert.warneke