pbpB
168
class B penicillin-binding protein 2B, Pbp2B, works together with [protein|8AB7D225DBEE6BD695A4A8A2384D2C029B571C57|ftsW] in the [wiki|divisome] in septal [wiki|cell wall synthesis]
locus
BSU_15160
Molecular weight
79.12 kDa
pI
9.23
function
septation, [wiki|cell division]
product
penicillin-binding protein 2B, member of the [wiki|divisome]
essential
yes
synonyms
pbpB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0768 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,581,947 → 1,584,097
Phenotypes of a mutant
essential [Pubmed|12682299]
deletion of the [wiki|PASTA domain]s is possible, results in impaired cell division and heat sensitivity [pubmed|32749956]
The protein
Catalyzed reaction/ biological activity
septal cell wall synthesis (this function can be taken over by [protein|5BBA2769A9C0EDB56625F008A55470C2E2885AA4|PBP3]) [pubmed|28792086]
assembly of the cell division complex (this is the reason for PbpB essentiality) [pubmed|28792086]
Protein family
[wiki|transpeptidase family] (according to UniProt)
[wiki|Domains]
contains two C-terminal [wiki|PASTA domain]s (aa 598-657, aa 658-714) (according to UniProt)
Structure
[PDB|1RP5] (PBP 2x from ''Streptococcus pneumoniae'', 32% identity) [Pubmed|14734544]
[AF|Q07868]
Paralogous protein(s)
[protein|38FD9805815BBDDD83789F0F402C0FB221B65FF4|spoVD]
[wiki|Localization]
cell membrane [Pubmed|23869552,20870765]
localizes to the division site, this depends on [protein|8AB7D225DBEE6BD695A4A8A2384D2C029B571C57|ftsW] [Pubmed|27895631,23895585]
extracellular (signal peptide) [Pubmed|18957862]
localizes to the sporulation septum during sporulation [Pubmed|15758244]
during vegetative growth: septal [pubmed|14731276]
Expression and Regulation
Operons
genes
[gene|A7131ACFA0CF5C222237C76E502D4AAD1F9BA3E4|mraZ]-[gene|1F894D6895CE13AA006CFE13A702E46C568F6E79|rsmH]-[gene|79712F4CF884A660C71B45B3397AEFA5C0AFA683|ftsL]-[gene|EBEFF9E0A524DCDA19382E5401B923B805E4C559|pbpB]
description
[Pubmed|8636036,8244929]
regulation
constitutively expressed [Pubmed|15758244]
Biological materials
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jeff Errington] lab
FLAG-tag construct
GP2003 ''pbpB-3xFLAG spec'' (based on [wiki|pGP1331]), available in [wiki|Jörg Stülke]'s lab
GFP fusion
3122 [gene|0467447B10EE7C1FDF86022AB6FBDBABFD32A9E5|trpC]2 [gene|EBEFF9E0A524DCDA19382E5401B923B805E4C559|pbpB]::pSG5061 (cat Pxyl-gfpa-[gene|EBEFF9E0A524DCDA19382E5401B923B805E4C559|pbpB]1-825) [pubmed|14731276], available in [wiki|Dirk Jan Scheffers]' lab and in the [http://bgsc.org BGSC] (due to essentiality of [gene|EBEFF9E0A524DCDA19382E5401B923B805E4C559|pbpB], the strain is xylose-dependent)
labs
[wiki|Jeff Errington], Newcastle University, UK [http://www.ncl.ac.uk/camb/staff/profile/jeff.errington homepage]
References
Reviews
Original Publications
Page visits: 7530
Time of last update: 2024-12-16 17:07:13
Author of last update: Jstuelk