yitA
168
sulfate adenylyltransferase
locus
BSU_10920
Molecular weight
43.09 kDa
pI
7.55
function
sulfate reduction and activation
product
sulfate adenylyltransferase
essential
no
ec
2.7.7.4
synonyms
yitA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2046 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,170,473 → 1,171,642
The protein
Catalyzed reaction/ biological activity
ATP + H+ + sulfate --> adenosine 5'-phosphosulfate + diphosphate (according to UniProt)
Protein family
sulfate adenylyltransferase family (with [protein|6CF2A9A517B641C44C0B46EF242BC5E668EA846B|sat], according to UniProt)
Structure
[PDB|1R6X] (from yeast, 41% identity) [pubmed|14983089]
[AF|O06736]
Paralogous protein(s)
[protein|6CF2A9A517B641C44C0B46EF242BC5E668EA846B|sat]
Expression and Regulation
Operons
genes
[gene|E5090587E23B284EF3B3D8F3EA5C44CB86EEB82E|yitB]-[gene|EBBA4432FF83B6F203C694E04111D45290BE157A|yitA]-[gene|FE2B31E5EBE6C4BA4FC4AAC485A00C97315E6AAB|yisZ]
description
[Pubmed|22383849]
regulation
expressed during sporulation in the mother cell ([protein|search|SigK]) [Pubmed|15699190,15383836]
sigma factors
[protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK]: sigma factor, [Pubmed|15699190,15383836], in [regulon|protein:24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK regulon]
Biological materials
Mutant
MGNA-A500 (yitA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/500 NBRP B. subtilis, Japan]
BKE10920 (Δ[gene|EBBA4432FF83B6F203C694E04111D45290BE157A|yitA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE10920 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACGTGTTCAACCTCCATT, downstream forward: _UP4_AAGCAAAACAGCGGGGAATT
BKK10920 (Δ[gene|EBBA4432FF83B6F203C694E04111D45290BE157A|yitA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK10920 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACGTGTTCAACCTCCATT, downstream forward: _UP4_AAGCAAAACAGCGGGGAATT
References
Page visits: 2213
Time of last update: 2024-12-26 03:15:36
Author of last update: Melvin.boenninger