yoaU
168
similar to transcriptional regulator ([wiki|LysR family])
locus
BSU_18760
Molecular weight
32.43 kDa
pI
6.56
function
unknown
product
transcriptional regulator ([wiki|LysR family])
essential
no
synonyms
yoaU
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0583 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,044,956 → 2,045,828
The protein
Protein family
[wiki|LysR family] (according to UniProt)
[wiki|Domains]
[wiki|HTH lysR-type domain] (aa 1-61) (according to UniProt)
Structure
[PDB|5Y2V] (from Synechocystis sp., 22% identity) [pubmed|29279392]
[AF|O34701]
Biological materials
Mutant
MGNA-A846 (yoaU::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/846 NBRP B. subtilis, Japan]
BKE18760 (Δ[gene|EBAC06AE1C91A51BD9F88386BA9633C4B51D073F|yoaU]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE18760 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGGTTATACCCCCAAT, downstream forward: _UP4_TGAATCAGTTATCGTGTTGC
BKK18760 (Δ[gene|EBAC06AE1C91A51BD9F88386BA9633C4B51D073F|yoaU]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK18760 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGGTTATACCCCCAAT, downstream forward: _UP4_TGAATCAGTTATCGTGTTGC
Expression vectors
pGP3931 (N-terminal Strep-tag, purification from E. coli, in [wiki|pGP172]), available in [wiki|Jörg Stülke]'s lab
References
Research papers
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Time of last update: 2024-11-20 12:56:55
Author of last update: Dennis.wicke