sacY

sacY
168

transcriptional antiterminator for sacB and sacX-sacY (acts at high sucrose concentrations)

Locus
BSU_38420
Molecular weight
32.31 kDa
Isoelectric point
5.88
Protein length
280 aaSequenceBlast
Gene length
843 bpSequenceBlast
Function
regulation of sucrose utilization
Product
transcriptional antiterminator
Essential
no
Synonyms
sacY, ipa-13r, sacS
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG3711 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,943,667 → 3,944,509
The protein
Catalyzed reaction/ biological activity
binding to the mRNA of sacB (sacB leader RNA) and the sacX-sacY operon (sacX leader RNA), causes transcription antitermination (in presence of sucrose)
Protein family
PRD-containing transcription factors
Domains
N-terminal RNA binding domain PubMed
2 x PRD (PtsI regulation domains) PubMed
2 PRD domains (aa 64-169, aa 170-280) (according to UniProt)
Structure
1H99 (PDB) (LicT PRDs, 36% identity) PubMed
1AUU (PDB) (RNA-binding domain)
P15401 (AlphaFold)
Paralogous protein(s)
LicT, SacT, GlcT
Expression and Regulation
Operons
Genes
sacX-sacY
Description
PubMed
Regulation
induction by sucrose (at high concentration) PubMed
Regulatory mechanism
DegU: activation, DegU-P, PubMed, in degU regulon
SacY: antitermination, in sacY regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Open in new tab

sacX→sacY

2025-03-12 12:01:41

ghost

109

71b8be28ad46269e23e5788d9176f974f9403a5a

B12CDDD44C2FA327FE195497E0BB83EF99EBC6D4

Biological materials
Mutant
GP425 (cat), available in Jörg Stülke's lab
BKE38420 (ΔsacY::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAGCTTTTTTGTCCTTTC,  downstream forward: _UP4_TGAGACAAACAAAAAACGCT
BKK38420 (ΔsacY::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAGCTTTTTTGTCCTTTC,  downstream forward: _UP4_TGAGACAAACAAAAAACGCT
Expression vectors
for expression, purification of RNA-binding domain in E. coli with N-terminal His-tag, in pWH844: pGP316, available in Jörg Stülke's lab
for expression, purification of RNA-binding domain in E. coli with N-terminal His-tag and thrombin cleavage site, in pGP570: pGP573, available in Jörg Stülke's lab
FLAG-tag construct
GP1222 (spc, based on pGP1331), available in Jörg Stülke's lab
GFP fusion
GP1226 (spc, based on pGP1870), available in Jörg Stülke's lab
Labs working on this gene/protein
Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France
References
Reviews
Loading
Original Publications
Loading

EB330AB3DC6C8C3EC72C4CAFA2BC59CC485EE344

Page visits: 4700

Time of last update: 2025-03-14 09:32:01

Author of last update: Jstuelk