zapA
168
positive modulator of [protein|41872E2EF00C79918DD077F2EF78F37E24FEB110|ftsZ] Z ring assembly and stability
locus
BSU_28610
Molecular weight
9.73 kDa
pI
7.17
function
control of Z-ring formation
product
Z-ring-associated protein, member of the [wiki|divisome]
essential
no
synonyms
zapA, yshA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG3027 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,925,640 → 2,925,897
Phenotypes of a mutant
[gene|EAD44AA7D72510B70308FD0C9F159DBEDAEBBB74|zapA] and [gene|B317D7E51824DD70EF84E4D5D7290D601BF4FAB6|ezrA] mutations are synthetically lethal, this can be suppressed by a [protein|41872E2EF00C79918DD077F2EF78F37E24FEB110|ftsZ]-K86E mutant protein [pubmed|33737746]
a [gene|1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA] [gene|EAD44AA7D72510B70308FD0C9F159DBEDAEBBB74|zapA] double mutant grows poorly, and the cells are filamentous (due to a defect in Z ring assembly) [Pubmed|24097947]
the defects of the [gene|1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA] [gene|EAD44AA7D72510B70308FD0C9F159DBEDAEBBB74|zapA] double mutant can be suppressed by inactivation of either [gene|D368988F85D8436DD424B0B4A1591BDF092A8EA7|gtaB], [gene|70412A747D1ED3E885126945DF02503667C13E5B|pgcA], or [gene|7A606B8E952AE8CA4F9A62008BA4B156725BB5B5|ugtP] [Pubmed|24097947]
The protein
Protein family
ZapA family (single member, according to UniProt)
Structure
[PDB|1W2E] (from ''Pseudomonas aeruginosa'') [Pubmed|15288790]
[AF|P94542]
[wiki|Localization]
colocalizes with the Z-ring [pubmed|38198550]
Expression and Regulation
Operons
genes
[gene|EAD44AA7D72510B70308FD0C9F159DBEDAEBBB74|zapA]-[gene|93726C423313C653C4F71CB107FA12F686959E41|yshB]-[gene|D3954F2BCF6CE66505E4F48FCB4A61EE8D24F6D3|polX]-[gene|F8E82D9AE514B31892FE4530532691274ADA682B|rqcU]-[gene|18CEF3F1EE2E95A009699DE370CBB44F025B7B40|yshE]
description
[Pubmed|22383849]
regulation
constitutively expressed [Pubmed|23701187]
Biological materials
Mutant
MGNA-B027 (yshA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1026 NBRP B. subtilis, Japan]
BKE28610 (Δ[gene|EAD44AA7D72510B70308FD0C9F159DBEDAEBBB74|zapA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE28610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACGTTTCTCCTCCATTCC, downstream forward: _UP4_CTTAAAGAAAAGGATTGAAC
BKK28610 (Δ[gene|EAD44AA7D72510B70308FD0C9F159DBEDAEBBB74|zapA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK28610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACGTTTCTCCTCCATTCC, downstream forward: _UP4_CTTAAAGAAAAGGATTGAAC
References
Reviews
Original Publications
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Time of last update: 2024-12-18 18:59:41
Author of last update: Jstuelk