deaD
168
DEAD-box RNA helicase
locus
BSU_39110
Molecular weight
53.85 kDa
pI
7.71
function
RNA helicase
product
DEAD-box RNA helicase
essential
no
synonyms
deaD, yxiN
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0513 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
4,015,987 → 4,017,426
The protein
Catalyzed reaction/ biological activity
RNA helicase
ATP + H2O --> ADP + H+ + phosphate (according to UniProt)
Protein family
[wiki|helicase family] (according to UniProt)
[wiki|DEAD-box RNA helicases] (according to UniProt)
[wiki|Domains]
[wiki|Helicase ATP-binding domain] (aa 33-203) (according to UniProt)
[wiki|Helicase C-terminal domain] (aa 214-374) (according to UniProt)
Structure
[PDB|5IVL] (from Geobacillus stearothermophilus, corresponds to aa 1 ... 373, 44% identity) [pubmed|28238534]
[PDB|2G0C] (RNA-binding domain, AA 404-479)
[PDB|2HJV] (second domain, AA 207-368)
[PDB|3MOJ] (RNA binding domain complexed with a fragment of 23S ribosomal RNA) [Pubmed|20673833]
[AF|P42305]
Paralogous protein(s)
[protein|0362D937D985846AEE4D0DEA57B62F915E8D35E5|yfmL], [protein|C83BD80DE0FD187D07B914F64696438ED5130107|cshB], [protein|E169F9711635457EA65FA71CCE9D7FF29DD8DF02|cshA]
Expression and Regulation
Operons
genes
[gene|D2790591E8C3B86BF91F6E1B6DADBA306D5EF758|yxiM]-[gene|EACD0C84AA3CE41EF05EAA3D25AA1153293EB562|deaD]
description
[Pubmed|10746760]
regulation
expression declines in the stationary phase [Pubmed|23175651]
Biological materials
Mutant
MGNA-B720 (deaD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1719 NBRP B. subtilis, Japan]
GP1052 (Δ[gene|EACD0C84AA3CE41EF05EAA3D25AA1153293EB562|deaD]::tet), available in [wiki|Jörg Stülke]'s lab
BKE39110 (Δ[gene|EACD0C84AA3CE41EF05EAA3D25AA1153293EB562|deaD]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE39110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTATAAACTCCTGTCTC, downstream forward: _UP4_AAATGATGAATGACCTGCTC
BKK39110 (Δ[gene|EACD0C84AA3CE41EF05EAA3D25AA1153293EB562|deaD]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK39110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTATAAACTCCTGTCTC, downstream forward: _UP4_AAATGATGAATGACCTGCTC
Expression vectors
for expression/ purification from ''B. subtilis'' with C-terminal Strep-tag, for [wiki|SPINE], expression from the native chromomsomal site: GP1065 (spc), available in [wiki|Jörg Stülke]'s lab
two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab, [pubmed|20572937]
FLAG-tag construct
GP1068 (spc, based on [wiki|pGP1331]), available in [wiki|Jörg Stülke]'s lab
labs
[wiki|Dagmar Klostermeier], Biozentrum Basel, Switzerland [http://www.biozentrum.unibas.ch/klostermeier/index.html homepage]
References
Reviews
Original Publications
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Time of last update: 2024-12-21 03:13:36
Author of last update: Jstuelk