tsaC
168
L-threonylcarbamoyl-AMP synthase, biosynthesis of the hypermodified base threonylcarbamoyladenosine (t(6)A) (together with [protein|6CB350C335603D5F4434679F9D740BFECA738599|tsaB]-[protein|30393F16CFE89E7BAD233A0BD27B77D679F7E409|tsaD]-[protein|E15BB518649B2B9CD44CEBBB4FA481E55BCE823C|tsaE])
locus
BSU_36950
Molecular weight
36.85 kDa
pI
5.82
function
tRNA modification
product
L-threonylcarbamoyl-AMP synthase
essential
no
ec
2.7.7.87
synonyms
tsaC, ipc-29d, ywlC
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0009 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,792,969 → 3,794,009
Phenotypes of a mutant
poor growth [pubmed|28189581]
poorly transformable [pubmed|28189581]
restores viability of a ''[gene|0136394A52759CEEDD8DE074ED74033C6FFB8435|smc]'' mutant on rich medium due to the induction of a [wiki|stringent response] [Pubmed|26539825]
The protein
Catalyzed reaction/ biological activity
conversion of L-threonine, bicarbonate/CO2 and ATP to give the intermediate L-threonylcarbamoyl-AMP (TC-AMP) and pyrophosphate as products [Pubmed|23072323]
biosynthesis of the hypermodified base threonylcarbamoyladenosine (t(6)A), a universal modification found at position 37 of ANN decoding tRNAs, which imparts a unique structure to the anticodon loop enhancing its binding to ribosomes in vitro (shown for the corresponding ''E. coli'' protein YrdC) [Pubmed|19287007]
ATP + hydrogencarbonate + L-threonine --> diphosphate + H2O + L-threonylcarbamoyladenylate (according to UniProt)
Protein family
SUA5 family (single member, according to UniProt)
[wiki|Domains]
YrdC-like domains (aa 18-205) (according to UniProt)
Structure
[PDB|1HRU] (the homolog from ''E. coli'') [Pubmed|19287007]
[AF|P39153]
Expression and Regulation
Operons
genes
[gene|EA155B3BEB9EAC97114058E23B6D8CDF8823A602|tsaC]
description
[Pubmed|22383849]
regulatory mechanism
[protein|6740108089F13116F200C15F35C2E7561E990FEB|dnaA]: repression, [Pubmed|17932079], in [regulon|protein:6740108089F13116F200C15F35C2E7561E990FEB|dnaA regulon]
Biological materials
Mutant
MGNA-A195 (ywlC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/195 NBRP B. subtilis, Japan]
BKE36950 (Δ[gene|EA155B3BEB9EAC97114058E23B6D8CDF8823A602|tsaC]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE36950 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTGACCAAACTCCTT, downstream forward: _UP4_TGAAAGCAGTCAGATGTTCT
BKK36950 (Δ[gene|EA155B3BEB9EAC97114058E23B6D8CDF8823A602|tsaC]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK36950 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTGACCAAACTCCTT, downstream forward: _UP4_TGAAAGCAGTCAGATGTTCT
References
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Time of last update: 2024-10-04 05:44:25
Author of last update: Melvin.boenninger