speB
168
N1-aminopropylagmatinase
locus
BSU_37490
Molecular weight
32.26 kDa
pI
4.77
function
[metabolite|spermidine], polyamine biosynthesis
product
N1-aminopropylagmatinase
essential
no
ec
3.5.3.11
synonyms
speB, ywhG
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0010 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,847,853 → 3,848,725
The protein
Catalyzed reaction/ biological activity
N1-[metabolite|aminopropylagmatine] + H2O --> [metabolite|spermidine] + [metabolite|urea] [https://doi.org/10.1101/2024.04.29.591726 bioRxiv]
Protein family
arginase family (with [protein|CD121BBF1DC206D1189ECF8B32456641A55C41D4|rocF] and [protein|FBB15E07B7134C57208EA82D5339708385DBC5FB|hutG], according to UniProt)
Structure
[PDB|3LHL] (from Clostridium difficile, 41% identity)
[AF|P70999]
Expression and Regulation
Operons
genes
[gene|4F3E96C741561746B4E0EEA5AE29DB124C2F7249|speE]-[gene|EA0AA0ECBC4A36A3F01F5876900FE51243D9AD07|speB]
description
[Pubmed|9723923]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|9723923], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-A522 (ywhG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/522 NBRP B. subtilis, Japan]
BKE37490 (Δ[gene|EA0AA0ECBC4A36A3F01F5876900FE51243D9AD07|speB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE37490 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTATAATACCCTCGCTT, downstream forward: _UP4_TAAGTAAACATCCAGACGAT
BKK37490 (Δ[gene|EA0AA0ECBC4A36A3F01F5876900FE51243D9AD07|speB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK37490 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTATAATACCCTCGCTT, downstream forward: _UP4_TAAGTAAACATCCAGACGAT
References
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Time of last update: 2025-02-10 17:01:53
Author of last update: Jstuelk