spoVG
168
RNA-binding regulatory protein, negative effector of asymetric septation at the onset of sporulation
locus
BSU_00490
Molecular weight
10.75 kDa
pI
5.11
function
cell division, control of sporulation initiation
product
negative effector of asymetric septation
essential
no
synonyms
spoVG
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2088 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
55,866 → 56,159
The protein
Protein family
SpoVG family (single member, according to UniProt)
Structure
[PDB|2IA9]
[AF|P28015]
Modification
phosphorylation on (Ser-66 OR Ser-67 OR Thr-68) [Pubmed|17218307]
Additional information
subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]
Expression and Regulation
Operons
genes
[gene|E9FF83FA90DCD7ECABFD67E95ECFAD3518DDC775|spoVG]
description
[Pubmed|408013]
regulation
repressed by [protein|5A6FBAE6553343092862CB79E150F934978C32A9|sinR] [Pubmed|16430695]
regulatory mechanism
[protein|5A6FBAE6553343092862CB79E150F934978C32A9|sinR]: repression, [Pubmed|16430695], in [regulon|protein:5A6FBAE6553343092862CB79E150F934978C32A9|sinR regulon]
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [Pubmed|2437099], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]
sigma factors
[protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH]: sigma factor, [Pubmed|408013], in [regulon|protein:DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH regulon]
Biological materials
Mutant
MGNA-A082 (spoVG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/82 NBRP B. subtilis, Japan]
GP2109 (''[gene|E9FF83FA90DCD7ECABFD67E95ECFAD3518DDC775|spoVG]''::''tet'', available in [wiki|Jörg Stülke]'s lab)
BKE00490 (Δ[gene|E9FF83FA90DCD7ECABFD67E95ECFAD3518DDC775|spoVG]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE00490 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAGTAGTTCACCACCTTT, downstream forward: _UP4_TAAAAAATAACCAAAAAGCA
BKK00490 (Δ[gene|E9FF83FA90DCD7ECABFD67E95ECFAD3518DDC775|spoVG]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK00490 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAGTAGTTCACCACCTTT, downstream forward: _UP4_TAAAAAATAACCAAAAAGCA
Expression vectors
pGP2163: expression of SpoVG-Strep by [wiki|pGP380] in ''B. subtilis'' suitable for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab
pGP2310: expression of SpoVG-Strep by [wiki|pGP382] in ''B. subtilis'' suitable for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab
References
Reviews
Original Publications
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Time of last update: 2025-04-07 02:01:30
Author of last update: Jstuelk