fni
168
isopentenyl diphosphate isomerase, last (8th) step in the MEP pathway of isoprenoid biosynthesis
locus
BSU_22870
Molecular weight
22.45 kDa
pI
6.1
function
MEP pathway of isoprenoid biosynthesis
product
isopentenyl diphosphate isomerase
essential
no
ec
5.3.3.2
synonyms
fni, ypgA, idi
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1304 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,393,602 → 2,394,651
The protein
Catalyzed reaction/ biological activity
isopentenyl diphosphate --> dimethylallyl diphosphate (according to UniProt)
Protein family
IPP isomerase type 2 family (single member, according to UniProt)
Structure
[PDB|1P0N] (complex with FMN)
[PDB|1P0K]
[AF|P50740]
[wiki|Localization]
cytoplasm (according to UniProt)
Expression and Regulation
Operons
genes
[gene|1ADB6E2C8DC82A242CFEFAD22443D14226F1BA60|cmk]-[gene|7C36409F155E4C91CEE902FE6937571BD0C50128|rpsA]-[gene|E91624382EC3DAABFB3123CDA818B4BEF745ACE6|fni]
description
[pubmed|22383849]
genes
[gene|7C36409F155E4C91CEE902FE6937571BD0C50128|rpsA]-[gene|E91624382EC3DAABFB3123CDA818B4BEF745ACE6|fni]
description
[pubmed|22383849]
genes
[gene|C194336ED96FA844FFE9FB43BE59ADC5D6DDB3BE|motI]-[gene|3459BA0CDAABC288ADF97B111F78C7EAC184840D|ypfB]-[gene|1ADB6E2C8DC82A242CFEFAD22443D14226F1BA60|cmk]-[gene|7C36409F155E4C91CEE902FE6937571BD0C50128|rpsA]-[gene|E91624382EC3DAABFB3123CDA818B4BEF745ACE6|fni]
description
[pubmed|22383849]
Biological materials
Mutant
MGNA-A420 (ypgA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/420 NBRP B. subtilis, Japan]
BKE22870 (Δ[gene|E91624382EC3DAABFB3123CDA818B4BEF745ACE6|fni]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE22870 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGCTCGAGTCACGTTTATCA, downstream forward: _UP4_TAAATGATAAAAGCCCAAAA
BKK22870 (Δ[gene|E91624382EC3DAABFB3123CDA818B4BEF745ACE6|fni]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK22870 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGCTCGAGTCACGTTTATCA, downstream forward: _UP4_TAAATGATAAAAGCCCAAAA
References
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Time of last update: 2024-12-20 08:09:44
Author of last update: Jstuelk