fpa

fpa
168

iron-sensing protein, antirepressor, inhibits the DNA-binding activity of [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur] in the absence of Fe2+

locus
BSU_14840
Molecular weight
10.60 kDa
pI
4.61
Protein length
Gene length
function
control of iron homeostasis
product
[protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur] protein antirepressor
essential
yes
synonyms
ylaN, furA

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG4838 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,552,412 → 1,552,693
Phenotypes of a mutant
essential [Pubmed|12682299], the mutant is viable if iron(III) is added to the medium [Pubmed|39251624,27238023]
The protein
Catalyzed reaction/ biological activity
binds Fe(II)
binds [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur] in the absence of iron to inhibit its DNA-binding activity and to induce genes of the [regulon|Fur regulon] for the [category|SW.1.3.3|Acquisition of iron] [pubmed|39251624]
Protein family
UPF0358 family (single member, according to UniProt)
Structure
[PDB|2ODM] (from Staphylococcus aureus, 64.6% identity) [Pubmed|17469204]
[AF|O07638]
Expression and Regulation
Operons
genes
[gene|E87DFC182D54EE96D4CA57607E734CDFB18E74EF|fpa]
description
[Pubmed|22383849]
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[gene|E87DFC182D54EE96D4CA57607E734CDFB18E74EF|fpa]

2024-09-27 20:55:14

ghost

67

9c386f35d52a9a5b91f355bfd78e7c060acf46ba

86720CC44AA5A190E597707744A4AD4E5A7A2AEA

Biological materials
Mutant
GP3324 (Δ[gene|E87DFC182D54EE96D4CA57607E734CDFB18E74EF|fpa]::''cat''), available in [wiki|Jörg Stülke]'s lab
GP3321 (Δ[gene|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]::''mls'' Δ[gene|E87DFC182D54EE96D4CA57607E734CDFB18E74EF|fpa]::''cat''), available in [wiki|Jörg Stülke]'s lab
BKE14840 (Δ[gene|E87DFC182D54EE96D4CA57607E734CDFB18E74EF|fpa]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE14840 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAAAGTATCCCTCCGTTCC,  downstream forward: _UP4_TAAATGATAAAACTCAAACT
BKK14840 (Δ[gene|E87DFC182D54EE96D4CA57607E734CDFB18E74EF|fpa]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK14840 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAAAGTATCCCTCCGTTCC,  downstream forward: _UP4_TAAATGATAAAACTCAAACT
Expression vectors
IPTG inducible expression of Strep-''ylaN'' in ''E. coli'': pGP2579 (in [wiki|pGP172]), available in [wiki|Jörg Stülke]'s lab
IPTG inducible expression of His-''ylaN'' in ''E. coli'': pGP2583 (in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab
pGP3897 constitutive overexpression of ''ylaN'', based on [wiki|pBQ200], available in [wiki|Jörg Stülke]'s lab
pGP3900 constitutive expression of Strep-''ylaN'' in ''B. subtilis'', based on [wiki|pGP380], available in [wiki|Jörg Stülke]'s lab
pGP3867 constitutive expression of ''ylaN''-Strep in ''B. subtilis'', based on [wiki|pGP382], available in [wiki|Jörg Stülke]'s lab
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), pGP3868, pGP3870, pGP3872, pGP3874, available in [wiki|Jörg Stülke]'s lab
lacZ fusion
pGP3593 (based on [wiki|pAC7]), available in [wiki|Jörg Stülke]'s lab
Antibody
available in [wiki|Jörg Stülke]'s lab
References
Reviews
36882621,37010421
Original Publications
17005971,17469204,22383849,23420519,27238023,28189581,32949815,36815589,39251624

E87DFC182D54EE96D4CA57607E734CDFB18E74EF

Page visits: 4730

Time of last update: 2024-10-04 22:10:38

Author of last update: Jstuelk