fpa
168
iron-sensing protein, antirepressor, inhibits the DNA-binding activity of [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur] in the absence of Fe2+
locus
BSU_14840
Molecular weight
10.60 kDa
pI
4.61
function
control of iron homeostasis
product
[protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur] protein antirepressor
essential
yes
synonyms
ylaN, furA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG4838 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,552,412 → 1,552,693
Phenotypes of a mutant
essential [Pubmed|12682299], the mutant is viable if iron(III) is added to the medium [Pubmed|39251624,27238023]
The protein
Catalyzed reaction/ biological activity
binds Fe(II) [pubmed|39440976]
binds [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur] in the absence of iron to inhibit its DNA-binding activity and to induce genes of the [regulon|Fur regulon] for the [category|SW.1.3.3|Acquisition of iron] [pubmed|39251624]
Protein family
UPF0358 family (single member, according to UniProt)
Structure
[PDB|2ODM] (from Staphylococcus aureus, 64.6% identity) [Pubmed|17469204]
[AF|O07638]
Expression and Regulation
Operons
Biological materials
Mutant
GP3324 (Δ[gene|E87DFC182D54EE96D4CA57607E734CDFB18E74EF|fpa]::''cat''), available in [wiki|Jörg Stülke]'s lab
GP3321 (Δ[gene|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]::''mls'' Δ[gene|E87DFC182D54EE96D4CA57607E734CDFB18E74EF|fpa]::''cat''), available in [wiki|Jörg Stülke]'s lab
BKE14840 (Δ[gene|E87DFC182D54EE96D4CA57607E734CDFB18E74EF|fpa]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE14840 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAAGTATCCCTCCGTTCC, downstream forward: _UP4_TAAATGATAAAACTCAAACT
BKK14840 (Δ[gene|E87DFC182D54EE96D4CA57607E734CDFB18E74EF|fpa]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK14840 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAAGTATCCCTCCGTTCC, downstream forward: _UP4_TAAATGATAAAACTCAAACT
Expression vectors
IPTG inducible expression of Strep-''ylaN'' in ''E. coli'': pGP2579 (in [wiki|pGP172]), available in [wiki|Jörg Stülke]'s lab
IPTG inducible expression of His-''ylaN'' in ''E. coli'': pGP2583 (in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab
pGP3897 constitutive overexpression of ''ylaN'', based on [wiki|pBQ200], available in [wiki|Jörg Stülke]'s lab
pGP3900 constitutive expression of Strep-''ylaN'' in ''B. subtilis'', based on [wiki|pGP380], available in [wiki|Jörg Stülke]'s lab
pGP3867 constitutive expression of ''ylaN''-Strep in ''B. subtilis'', based on [wiki|pGP382], available in [wiki|Jörg Stülke]'s lab
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), pGP3868, pGP3870, pGP3872, pGP3874, available in [wiki|Jörg Stülke]'s lab
lacZ fusion
pGP3593 (based on [wiki|pAC7]), available in [wiki|Jörg Stülke]'s lab
Antibody
available in [wiki|Jörg Stülke]'s lab
References
Reviews
Original Publications
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Time of last update: 2024-11-20 19:30:00
Author of last update: Jstuelk