yabT
168
Ser/Thr kinase, controls [category|SW.3.3.1|Translation] and DNA integrity during spore development
locus
BSU_00660
Molecular weight
37.51 kDa
pI
9.94
function
control of DNA integrity during spore development
product
Ser/Thr kinase
essential
no
synonyms
yabT
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0515 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
73,809 → 74,825
Phenotypes of a mutant
the mutant tends to acquire suppressor mutations that result in improved growth [Pubmed|28189581]
increased sensitivity to DNA damage during spore development [Pubmed|23634894]
The protein
Catalyzed reaction/ biological activity
phosphorylation of [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA] on Ser-2 [Pubmed|23634894]
phosphorylation of [gene|E771685D6D41D48E0C0AF77F15B4F380820FCDC2|EF-Tu] on Thr-63 during [wiki|sporulation] [Pubmed|26056311]
phosphorylation of [protein|A039228A3805F4E7C5C2AE31C7DB0808562E88E3|yabA] on Thr-71 [pubmed|29619013]
ATP + L-seryl-[protein] --> ADP + H+ + O-phospho-L-seryl-[protein] (according to UniProt)
ATP + L-threonyl-[protein] --> ADP + H+ + O-phospho-L-threonyl-[protein] (according to UniProt)
Protein family
[wiki|protein kinase superfamily] (according to UniProt)
[wiki|Ser/Thr protein kinase family] (according to UniProt)
[wiki|Domains]
[wiki|Protein kinase domain] (aa 28-286) (according to UniProt)
Structure
[PDB|6G4J] [pubmed|30671027]
[AF|P37562]
Effectors of protein activity
autophosphorylation is stimulated by non-specific binding to DNA [Pubmed|23634894]
[wiki|Localization]
colocalizes strongly with the septal membrane separating the mother cells from the forespore [Pubmed|23634894]
Expression and Regulation
Operons
genes
[gene|7C8DFE00A2B2B30CC8BEB35055D92CC2E4128F3A|spoIIE]-[gene|C8AA53889FC8B389E1D921B762BD78990C7A8E57|yabS]-[gene|E86A96B832351FF513DD9853EAD8998CC44C9951|yabT]-[gene|473F93EA5ADB78CD23C9C054B5D6B739078DDEEB|tilS]-[gene|B7BFC170EEC88DEC6CF932F80805D3F2C0BD3738|hprT]-[gene|4E7B9426CED372AA8A321A147116A3A589FBF20C|ftsH]
description
[pubmed|22383849]
regulation
expressed early during sporulation in the forespore ([protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]) [Pubmed|16497325]
sigma factors
[protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]: sigma factor, [Pubmed|16497325], in [regulon|protein:CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF regulon]
Biological materials
Mutant
MGNA-B921 (yabT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1920 NBRP B. subtilis, Japan]
GP577 (erm), available in [wiki|Jörg Stülke]'s lab
BKE00660 (Δ[gene|E86A96B832351FF513DD9853EAD8998CC44C9951|yabT]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE00660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTGATTGTCGTACCCGGCT, downstream forward: _UP4_TGAATGGTGCAAACTGCAGA
BKK00660 (Δ[gene|E86A96B832351FF513DD9853EAD8998CC44C9951|yabT]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK00660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTGATTGTCGTACCCGGCT, downstream forward: _UP4_TGAATGGTGCAAACTGCAGA
Expression vectors
purification from ''B. subtilis'' with N-terminal Strep-tag, for [wiki|SPINE], in [wiki|pGP380]: pGP391, available in [wiki|Jörg Stülke]'s lab
purification from ''E. coli'' with N-terminal Strep-tag, in [wiki|pGP172]: pGP823, available in [wiki|Jörg Stülke]'s lab, [pubmed|20389117]
purification from ''E. coli'' with N-terminal His-tag, in [wiki|pWH844]: pGP1408, available in [wiki|Jörg Stülke]'s lab
lacZ fusion
translational lacZ fusion (in [wiki|pAC7]): pGP831, available in [wiki|Jörg Stülke]'s lab
References
Reviews
Original Publications
Page visits: 4344
Time of last update: 2024-12-21 08:22:01
Author of last update: Christoph.elfmann