pyrAA
168
carbamoyl-phosphate synthetase (glutaminase subunit)
locus
BSU_15510
Molecular weight
39.96 kDa
pI
5.82
function
pyrimidine biosynthesis
product
carbamoyl-phosphate synthetase (glutaminase subunit)
essential
no
ec
6.3.5.5
synonyms
pyrAA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0505 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,622,657 → 1,623,751
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The protein
Catalyzed reaction/ biological activity
2 ATP + H2O + hydrogencarbonate + L-glutamine --> 2 ADP + carbamoyl phosphate + 2 H+ + L-glutamate + phosphate (according to UniProt)
Protein family
CarA family (with [protein|4202EAE5D0B2A718382092423DD99E35FDE81218|carA], according to UniProt)
[wiki|Domains]
[wiki|Glutamine amidotransferase type-1 domain] (aa 171-356) (according to UniProt)
Structure
[PDB|1JDB] (from ''E. coli'', 47% identity) [Pubmed|10089390]
[AF|P25993]
Paralogous protein(s)
[protein|4202EAE5D0B2A718382092423DD99E35FDE81218|carA]
Expression and Regulation
Operons
genes
[gene|6D9CEDC7737CCFA838EBC142CD735E066C950AD2|pyrR]-[gene|A5C26E10085868A0A193C450DF58D54C636B7C2D|pyrP]-[gene|B83462A50FDDD20FE9BD4681824E30AAD10F506B|pyrB]-[gene|CA16FD3507DCCADB1E01DF25B42C3405C9205B05|pyrC]-[gene|E7741ED6F15044900BDC5F0584246469D2D4D586|pyrAA]-[gene|D57DC8A53E52BC4A2EA894E3C9FD983592882918|pyrAB]-[gene|C20A05AE4CE8AEE0DA767334935CC64106A7FCCB|pyrK]-[gene|E533BEF28579CD04B24E2C728737269E35224450|pyrD]-[gene|5D0D0D2413377FCA92440334E92B5A27A5963D9E|pyrF]-[gene|0197A037D2AF049B295E1DA60187D4614B2BAC51|pyrE]
description
[Pubmed|8206849]
regulation
induced in the absence of uridine nucleotides ([protein|search|PyrR]) [Pubmed|8206849]
regulatory mechanism
[protein|6D9CEDC7737CCFA838EBC142CD735E066C950AD2|pyrR]: termination, via [wiki|RNA switch], in [regulon|protein:6D9CEDC7737CCFA838EBC142CD735E066C950AD2|pyrR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|1709162], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
BKE15510 (Δ[gene|E7741ED6F15044900BDC5F0584246469D2D4D586|pyrAA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15510 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GTTTTCCAGTACTAATCGTC, downstream forward: _UP4_ACAGAGAAAGAAGGGGAAGC
BKK15510 (Δ[gene|E7741ED6F15044900BDC5F0584246469D2D4D586|pyrAA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15510 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GTTTTCCAGTACTAATCGTC, downstream forward: _UP4_ACAGAGAAAGAAGGGGAAGC
GP3716 ([gene|E7741ED6F15044900BDC5F0584246469D2D4D586|pyrAA]::aphA3 trpC2) available in the [wiki|Stülke] lab
GP3719 ([gene|search|pyrAAAB]::aphA3 trpC2) available in the [wiki|Stülke] lab
GP3721 ([gene|search|pyrAAAB]::cat trpC2) available in the [wiki|Stülke] lab
Expression vectors
pGP3772: expression of Strep-''pyrAA'' by [wiki|pGP380] in ''B. subtilis'' suitable for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab
References
Reviews
Original Publications
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Time of last update: 2024-09-10 14:46:08
Author of last update: Robert.warneke