malP
168
maltose-specific permease of the [wiki|phosphotransferase systems|phosphotransferase system], EIICB of the [category|SW.1.2.2|PTS]
locus
BSU_08200
Molecular weight
57.89 kDa
pI
8.87
function
maltose uptake and phosphorylation
product
maltose-specific [category|SW.1.2.2|PTS], EIICB
essential
no
ec
2.7.1.69
synonyms
malP, glvC
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1264 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
892,215 → 893,798
Phenotypes of a mutant
no growth on maltose as single carbon source [Pubmed|10627040]
The protein
Catalyzed reaction/ biological activity
D-maltose + Nπ-phospho-L-histidyl-[protein] --> α-maltose 6'-phosphate + L-histidyl-[protein] (according to UniProt)
Protein family
[category|SW.1.2.2|PTS] permease, glucose family [Pubmed|10627040]
[wiki|Domains]
[wiki|PTS EIIB domain] type-1 (aa 449–527) (according to UniProt)
[wiki|PTS EIIC domain] type-1 (aa 1-418) (according to UniProt)
Structure
[PDB|6BVG] (EIIC domain from B. cereus, corresponds to aa 6 - 419, 27% identity) [pubmed|29784777]
[AF|P54715]
[wiki|Localization]
cell membrane (according to UniProt)
Expression and Regulation
Operons
genes
[gene|C650160C9E5B5F8587CA9E80A57BCCE97D701037|malA]-[gene|AD72489DA260F07B97A213F1F0FBA7CED9471817|glvR]-[gene|E6C7733A12AF9A5322EED444E4A4FF1605A8B20F|malP]
description
[Pubmed|11489864]
regulation
induction by maltose ([protein|search|GlvR]) [Pubmed|11489864]
regulatory mechanism
[protein|AD72489DA260F07B97A213F1F0FBA7CED9471817|glvR]: activation, [Pubmed|11489864], in [regulon|protein:AD72489DA260F07B97A213F1F0FBA7CED9471817|glvR regulon]
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|11489864], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|11489864], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-C291 (glvC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2289 NBRP B. subtilis, Japan]
GP110 (spc), available in [wiki|Stülke] lab
BKE08200 (Δ[gene|E6C7733A12AF9A5322EED444E4A4FF1605A8B20F|malP]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE08200 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGCATCATACCAGAACCCC, downstream forward: _UP4_TAAAGACTGCCCTCCTTTTC
BKK08200 (Δ[gene|E6C7733A12AF9A5322EED444E4A4FF1605A8B20F|malP]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK08200 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGCATCATACCAGAACCCC, downstream forward: _UP4_TAAAGACTGCCCTCCTTTTC
References
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Time of last update: 2024-10-03 07:31:56
Author of last update: Melvin.boenninger