forC1
168
formate:menadione oxidoreductase (with [protein|01C43D05A53BF4B0F505D9C9CCAAFA1AF9E94F87|forE1]), catalytic subunit, general stress protein, important for survival of salt and ethanol stress
locus
BSU_12160
Molecular weight
109.58 kDa
pI
5.18
function
unknown
product
formate:menadione oxidoreductase
essential
no
synonyms
yjgC
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG3383 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,285,591 → 1,288,548
The protein
Protein family
C-terminal part: [wiki|prokaryotic molybdopterin-containing oxidoreductase family] (according to UniProt)
[wiki|Domains]
2Fe-2S ferredoxin-type domain (aa 3-79) (according to UniProt)
4Fe-4S His(Cys)3-ligated-type domain (aa 79-119) (according to UniProt)
2 [wiki|4Fe-4S ferredoxin-type domain]s (aa 139-170, aa 182-211) (according to UniProt)
[wiki|4Fe-4S Mo/W bis-MGD-type domain] (aa 258-314) (according to UniProt)
[wiki|Cofactors]
4 4Fe-4S cluster [pubmed|34748728,29292548]
1 2FE-2S cluster [pubmed|34748728]
molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) [pubmed|34748728]
menachinone-7 [pubmed|34748728]
Structure
[PDB|6TG9] (from Rhodobacter capsulatus, 35% identity) [pubmed|32313256]
[PDB|2FUG] (from Thermus thermophilus, 23% identity) [pubmed|16469879]
[AF|O34720]
Paralogous protein(s)
[protein|A4304213CFC152B601B2EED2B9B3A0A3AB4E6A81|forC2]
Expression and Regulation
Operons
genes
[gene|E54F39E72DC4881E886159607C2B424A41DE0D01|forC1]-[gene|01C43D05A53BF4B0F505D9C9CCAAFA1AF9E94F87|forE1]
description
[Pubmed|11544224]
regulation
induced by stress ([protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]) [Pubmed|11544224,15805528]
sigma factors
[protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|11544224,15805528], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]
Biological materials
Mutant
MGNA-A354 (yjgC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/354 NBRP B. subtilis, Japan]
BKE12160 (Δ[gene|E54F39E72DC4881E886159607C2B424A41DE0D01|forC1]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE12160 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCCATTCCCCTTTCTT, downstream forward: _UP4_GCTGTGAAAAGGGGGATGGG
BKK12160 (Δ[gene|E54F39E72DC4881E886159607C2B424A41DE0D01|forC1]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK12160 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCCATTCCCCTTTCTT, downstream forward: _UP4_GCTGTGAAAAGGGGGATGGG
References
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Time of last update: 2024-12-30 18:00:06
Author of last update: Jstuelk