yqhB
168
similar to magnesium exporter, general stress protein, required for protection against oxidative stress
locus
BSU_24750
Molecular weight
49.79 kDa
pI
5.54
function
protection against stress conditions
product
unknown
essential
no
synonyms
yqhB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1253 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,561,585 → 2,562,913
The protein
Protein family
[wiki|UPF0053 family](according to UniProt)
[wiki|Domains]
[wiki|CNNM transmembrane domain] (aa 7-210) (according to UniProt)
[wiki|DUF21 domain] (14-210)
2 [wiki|CBS domain]s (aa 229-290, aa 295-355) (according to UniProt)
Structure
[PDB|4HG0] (CorC from E. coli, corresponds to the [wiki|CBS domain]s and the C-terminal transporter-associated domain)
[AF|P54505]
Paralogous protein(s)
[protein|45F03AB13292BBF0554785C7C02FE29033EDD742|yrkA], [protein|A459410312A926365B45CEB4694DE399B38820A2|yugS], [protein|B2E9D026B668C8530C406353E3400059388566FB|yhdT], [protein|BE0777DE7C40825D4DB7252EA84AAB3892578529|mpfA]
[wiki|Localization]
cell membrane (according to UniProt)
Expression and Regulation
Operons
genes
[gene|E53B8D437B848B637AEC8C1BB9428EC8B6EB280E|yqhB]
description
[Pubmed|16267290]
regulation
induced by stress ([protein|search|SigB]) [Pubmed|15805528]
regulatory mechanism
[protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA]: repression, [Pubmed|16267290], in [regulon|protein:D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA regulon]
sigma factors
[protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|15805528], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]
Biological materials
Mutant
MGNA-C474 (yqhB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2472 NBRP B. subtilis, Japan]
BKE24750 (Δ[gene|E53B8D437B848B637AEC8C1BB9428EC8B6EB280E|yqhB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE24750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCACCTCCCTTTTCCGA, downstream forward: _UP4_TAGCCCGTGAAAGGCTGTTC
BKK24750 (Δ[gene|E53B8D437B848B637AEC8C1BB9428EC8B6EB280E|yqhB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK24750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCACCTCCCTTTTCCGA, downstream forward: _UP4_TAGCCCGTGAAAGGCTGTTC
Expression vectors
pGP2935 (N-terminal His-tag, purification from ''E. coli'', in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab
pGP3467 (223-359aa) (N-terminal 6xHis-tag, purification from E. coli, in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab
pGP3472 (223-359aa) (N-terminal 6xHis-SUMO-tag, purification from E. coli, in pET-SUMO), available in [wiki|Jörg Stülke]'s lab
References
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Time of last update: 2024-10-06 00:20:40
Author of last update: LKrueger