pyrD
168
dihydroorotic acid dehydrogenase (catalytic subunit)
locus
BSU_15540
Molecular weight
32.94 kDa
pI
4.94
function
pyrimidine biosynthesis
product
dihydroorotic acid dehydrogenase (catalytic subunit)
essential
no
ec
1.3.3.1
synonyms
pyrD, pyrDI
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0167 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,627,718 → 1,628,653
The protein
Catalyzed reaction/ biological activity
(S)-dihydroorotate + NAD+ --> H+ + NADH + orotate (according to UniProt)
Protein family
dihydroorotate dehydrogenase family (single member, according to UniProt)
[wiki|Cofactors]
FMN [pubmed|11188687]
Structure
[PDB|1EP1] (from ''Lactococcus lactis'', 68% identity) [Pubmed|11188687]
[AF|P25996]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|6D9CEDC7737CCFA838EBC142CD735E066C950AD2|pyrR]-[gene|A5C26E10085868A0A193C450DF58D54C636B7C2D|pyrP]-[gene|B83462A50FDDD20FE9BD4681824E30AAD10F506B|pyrB]-[gene|CA16FD3507DCCADB1E01DF25B42C3405C9205B05|pyrC]-[gene|E7741ED6F15044900BDC5F0584246469D2D4D586|pyrAA]-[gene|D57DC8A53E52BC4A2EA894E3C9FD983592882918|pyrAB]-[gene|C20A05AE4CE8AEE0DA767334935CC64106A7FCCB|pyrK]-[gene|E533BEF28579CD04B24E2C728737269E35224450|pyrD]-[gene|5D0D0D2413377FCA92440334E92B5A27A5963D9E|pyrF]-[gene|0197A037D2AF049B295E1DA60187D4614B2BAC51|pyrE]
description
[Pubmed|8206849]
regulation
induced in the absence of uridine nucleotides ([protein|search|PyrR]) [Pubmed|8206849]
regulatory mechanism
[protein|6D9CEDC7737CCFA838EBC142CD735E066C950AD2|pyrR]: termination, via [wiki|RNA switch], in [regulon|protein:6D9CEDC7737CCFA838EBC142CD735E066C950AD2|pyrR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|1709162], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
1A806 ( ''pyrD''::''cat''), [Pubmed|15109830], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A806&Search=1A806 BGSC]
1A807 ( ''pyrD''::''kan''), [Pubmed|15109830], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A807&Search=1A807 BGSC]
BKE15540 (Δ[gene|E533BEF28579CD04B24E2C728737269E35224450|pyrD]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TCCCGGCAATTTCACCTCTA, downstream forward: _UP4_GAATGCATCGGAAGGAGCTG
BKK15540 (Δ[gene|E533BEF28579CD04B24E2C728737269E35224450|pyrD]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TCCCGGCAATTTCACCTCTA, downstream forward: _UP4_GAATGCATCGGAAGGAGCTG
References
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Time of last update: 2025-04-26 03:39:12
Author of last update: Melvin.boenninger