pyrD

pyrD
168

dihydroorotic acid dehydrogenase (catalytic subunit)

Locus
BSU_15540
Molecular weight
32.94 kDa
Isoelectric point
4.94
Protein length
311 aaSequenceBlast
Gene length
936 bpSequenceBlast
Function
pyrimidine biosynthesis
Product
dihydroorotic acid dehydrogenase (catalytic subunit)
Essential
no
E.C.
1.3.3.1
Synonyms
pyrD, pyrDI
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0167 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,627,718 → 1,628,653
The protein
Catalyzed reaction/ biological activity
(S)-dihydroorotate + NAD+ --> H+ + NADH + orotate (according to UniProt)
Protein family
dihydroorotate dehydrogenase family (single member, according to UniProt)
Cofactors
FMN PubMed
Structure
1EP1 (PDB) (from Lactococcus lactis, 68% identity) PubMed
P25996 (AlphaFold)
Localization
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
Genes
pyrR-pyrP-pyrB-pyrC-pyrAA-pyrAB-pyrK-pyrD-pyrF-pyrE
Description
PubMed
Regulation
induced in the absence of uridine nucleotides (PyrR) PubMed
Regulatory mechanism
PyrR: termination, via RNA switch, in pyrR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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pyrR→pyrE

2025-04-07 02:33:30

ghost

112

3b978222b0b6a5230a2e7701ac35d1e35cc85307

0A8FD5CB6489EDD8CB2074130B763DCAC1F24654

Biological materials
Mutant
1A806 ( pyrD::cat), PubMed, available at BGSC
1A807 ( pyrD::kan), PubMed, available at BGSC
BKE15540 (ΔpyrD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TCCCGGCAATTTCACCTCTA,  downstream forward: _UP4_GAATGCATCGGAAGGAGCTG
BKK15540 (ΔpyrD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TCCCGGCAATTTCACCTCTA,  downstream forward: _UP4_GAATGCATCGGAAGGAGCTG
References
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E533BEF28579CD04B24E2C728737269E35224450

Page visits: 4320

Time of last update: 2025-04-12 06:16:18

Author of last update: Melvin.boenninger