yitB
168
phospho-adenylylsulfate sulfotransferase
locus
BSU_10930
Molecular weight
27.52 kDa
pI
5.77
function
sulfate reduction and activation
product
phospho-adenylylsulfate sulfotransferase
essential
no
ec
1.8.4.8
synonyms
yitB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0175 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,171,755 → 1,172,465
The protein
Catalyzed reaction/ biological activity
[thioredoxin]-disulfide + adenosine 3',5'-bisphosphate + 2 H+ + sulfite --> 3'-phosphoadenylyl sulfate + [thioredoxin]-dithiol (according to UniProt)
Protein family
PAPS reductase family (with [protein|51EB09C122A7166D662AE72842A44EC5FBA03134|cysH], according to UniProt)
Structure
[PDB|2OQ2] (from yeast, 33% identity) [pubmed|18991405]
[AF|O06737]
Paralogous protein(s)
[protein|51EB09C122A7166D662AE72842A44EC5FBA03134|cysH]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|E5090587E23B284EF3B3D8F3EA5C44CB86EEB82E|yitB]-[gene|EBBA4432FF83B6F203C694E04111D45290BE157A|yitA]-[gene|FE2B31E5EBE6C4BA4FC4AAC485A00C97315E6AAB|yisZ]
description
[Pubmed|22383849]
regulation
expressed during sporulation in the mother cell ([protein|search|SigK]) [Pubmed|15699190,15383836]
sigma factors
[protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK]: sigma factor, [Pubmed|15699190,15383836], in [regulon|protein:24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK regulon]
Biological materials
Mutant
MGNA-A504 (yitB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/504 NBRP B. subtilis, Japan]
BKE10930 (Δ[gene|E5090587E23B284EF3B3D8F3EA5C44CB86EEB82E|yitB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE10930 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGTTTTCCCCCCGTTTG, downstream forward: _UP4_TAGCGGATGGCAGGTTCTTG
BKK10930 (Δ[gene|E5090587E23B284EF3B3D8F3EA5C44CB86EEB82E|yitB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK10930 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGTTTTCCCCCCGTTTG, downstream forward: _UP4_TAGCGGATGGCAGGTTCTTG
References
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Time of last update: 2025-03-15 22:49:33
Author of last update: Melvin.boenninger