mcpB

mcpB
168

membrane-bound chemotaxis receptor, methyl-accepting chemotaxis protein, receptor for asparagine as well as ethanol and other short-chain alcohols, acts also as pH sensor, responds to increasing pH as an attractant signal

locus
BSU_31260
Molecular weight
71.70 kDa
pI
5.13
Protein length
Gene length
function
control of chemotaxis, sensing of different compounds
product
methyl-accepting chemotaxis protein
essential
no
synonyms
mcpB

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0840 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,210,445 → 3,212,433
The protein
[wiki|Domains]
[wiki|Cache domain] (aa 153-229) (according to UniProt)
[wiki|HAMP domain] (aa 304-356) (according to UniProt)
[wiki|Methyl-accepting transducer domain] (aa 375-611) (according to UniProt)
Structure
[PDB|6S1K] (from E. coli, corresponds to aa 280 ... 545, 33% identity) [pubmed|31925330]
[AF|P39215]
Modification
methylated at residues Gln371, Glu630, and Glu637 [Pubmed|20864474]
Effectors of protein activity
asparagine (binds to the part of McpB that is exposed to the exterior) [Pubmed|19864420]
Kinetic information
K(D) for asparagine: 14 myM [Pubmed|19864420]
Paralogous protein(s)
[protein|F02E3080214A327E131ECDE0DBC52C57EFDFE2CE|mcpA], [protein|4CD44AD8FE68516C84889EAA2137828E06B8A30B|tlpB], [protein|1DE26CDEE952142C1303C822F0E2A63AE09F721B|tlpA], [protein|8333CD46F704F03A22482CAA98DEFCC945362A10|mcpC]
[wiki|Localization]
cell membrane [Pubmed|18763711,21515776]
localized at the cell poles, but in the presence of high asparagine concentration there is a reversible re-localization to the sides of the cell (lateral clusters) [Pubmed|21098025]
Expression and Regulation
Operons
genes
[gene|E4E1020B799A1B403BDB4847B91B02D64D161AF3|mcpB]
description
[Pubmed|8188684]
sigma factors
[protein|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD]: sigma factor, [Pubmed|8188684], in [regulon|protein:7024E4162A6D827069F882FDEACA696EBC05DD40|sigD regulon]
additional information
in minimal medium, McpB is present with 6,200 /- 1,900 molecules per cell [PubMed|21515776]
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[gene|E4E1020B799A1B403BDB4847B91B02D64D161AF3|mcpB]

2024-12-10 09:05:25

ghost

80

8febabfc1752d84f4e3d93576a6fb1c550681196

8F9AC414A1DD8DE4EB645DDF2A89933730E88557

Biological materials
Mutant
BKE31260 (Δ[gene|E4E1020B799A1B403BDB4847B91B02D64D161AF3|mcpB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE31260 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTCACTTGCTCCTTCAG,  downstream forward: _UP4_TAAAAACCGAAAAACAGCGC
BKK31260 (Δ[gene|E4E1020B799A1B403BDB4847B91B02D64D161AF3|mcpB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK31260 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTCACTTGCTCCTTCAG,  downstream forward: _UP4_TAAAAACCGAAAAACAGCGC
References
Reviews
31792011,35323015
Original Publications
36815589,11722727,10825179,8251536,12909020,10196193,15544802,6137212,9194713,2505839,8188684,2105313,12123464,14731274,20864474,18763711,19864420,21098025,21515776,25039821,27899502,31685537,31925330,33024039

E4E1020B799A1B403BDB4847B91B02D64D161AF3

Page visits: 2694

Time of last update: 2024-12-21 16:31:51

Author of last update: Jstuelk