mcpB
168
membrane-bound chemotaxis receptor, methyl-accepting chemotaxis protein, receptor for asparagine as well as ethanol and other short-chain alcohols, acts also as pH sensor, responds to increasing pH as an attractant signal
locus
BSU_31260
Molecular weight
71.70 kDa
pI
5.13
function
control of chemotaxis, sensing of different compounds
product
methyl-accepting chemotaxis protein
essential
no
synonyms
mcpB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0840 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,210,445 → 3,212,433
The protein
[wiki|Domains]
[wiki|Cache domain] (aa 153-229) (according to UniProt)
[wiki|HAMP domain] (aa 304-356) (according to UniProt)
[wiki|Methyl-accepting transducer domain] (aa 375-611) (according to UniProt)
Structure
[PDB|6S1K] (from E. coli, corresponds to aa 280 ... 545, 33% identity) [pubmed|31925330]
[AF|P39215]
Modification
methylated at residues Gln371, Glu630, and Glu637 [Pubmed|20864474]
Effectors of protein activity
asparagine (binds to the part of McpB that is exposed to the exterior) [Pubmed|19864420]
Kinetic information
K(D) for asparagine: 14 myM [Pubmed|19864420]
Paralogous protein(s)
[protein|F02E3080214A327E131ECDE0DBC52C57EFDFE2CE|mcpA], [protein|4CD44AD8FE68516C84889EAA2137828E06B8A30B|tlpB], [protein|1DE26CDEE952142C1303C822F0E2A63AE09F721B|tlpA], [protein|8333CD46F704F03A22482CAA98DEFCC945362A10|mcpC]
[wiki|Localization]
cell membrane [Pubmed|18763711,21515776]
localized at the cell poles, but in the presence of high asparagine concentration there is a reversible re-localization to the sides of the cell (lateral clusters) [Pubmed|21098025]
Expression and Regulation
Operons
genes
[gene|E4E1020B799A1B403BDB4847B91B02D64D161AF3|mcpB]
description
[Pubmed|8188684]
sigma factors
[protein|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD]: sigma factor, [Pubmed|8188684], in [regulon|protein:7024E4162A6D827069F882FDEACA696EBC05DD40|sigD regulon]
additional information
in minimal medium, McpB is present with 6,200 /- 1,900 molecules per cell [PubMed|21515776]
Biological materials
Mutant
BKE31260 (Δ[gene|E4E1020B799A1B403BDB4847B91B02D64D161AF3|mcpB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE31260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCACTTGCTCCTTCAG, downstream forward: _UP4_TAAAAACCGAAAAACAGCGC
BKK31260 (Δ[gene|E4E1020B799A1B403BDB4847B91B02D64D161AF3|mcpB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK31260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCACTTGCTCCTTCAG, downstream forward: _UP4_TAAAAACCGAAAAACAGCGC
References
Reviews
Original Publications
Page visits: 2619
Time of last update: 2024-10-08 23:05:13
Author of last update: Jstuelk