accD
168
acetyl-CoA carboxylase (beta subunit)
locus
BSU_29210
Molecular weight
28.73 kDa
pI
5.34
function
production of malonyl-CoA, the substrate for fatty acid biosynthesis
product
acetyl-CoA carboxylase (beta subunit))
essential
yes
ec
6.4.1.2
synonyms
accD, yttI
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0777 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,988,693 → 2,989,565
Phenotypes of a mutant
essential [Pubmed|12682299]
enhanced colony wrinkling upon knockdown [pubmed|36069446]
additional information
[gene|A8EEC896B279CFDDE9C3A6F146A04EC822E33A4F|ponA] [gene|59A7B26F810D7FEB07C176A8B2ECF6B83803DE49|ecsA], [gene|A8EEC896B279CFDDE9C3A6F146A04EC822E33A4F|ponA] [gene|3DEAC421B4B173C6BBC700E57790751B7AFDF319|sigI], and [gene|A8EEC896B279CFDDE9C3A6F146A04EC822E33A4F|ponA] [gene|CB50289535EA537F63BADD459BD11AA7759A6658|rasP] double mutants have a growth defect that can be suppressed by reduced fatty acid synthesis (mutations in [gene|22B3039DF4D106F121DBC765F49878C9B324FDB2|accA], [gene|ADD1CE87C1C5BA44FFF5499EA5E155C4C53A1C22|accB], [gene|1DFFEB155028A5C6B4B344769EF7DAAC14983322|accC], [gene|E4997526CC534E38413030E90F7A135ECF65DF40|accD] or downregulation of the [wiki|FapR regulon] due to specific mutations in [gene|FCDE900167AE36377979E0CF7BC33C7F351B95D3|fapR]) [pubmed|37017514]
The protein
Catalyzed reaction/ biological activity
acetyl-CoA + N6-carboxybiotinyl-L-lysyl-[protein] --> malonyl-CoA + N6-biotinyl-L-lysyl-[protein] (according to UniProt)
Protein family
AccD/PCCB family (with [protein|2192474E13EAF6D2CF97CB957864FC89ED0118E0|yngE] and [protein|B6A05AD90A4AA553A8CCD8FFF504F5AD204ABAFB|yqjD], according to UniProt)
[wiki|Domains]
CoA carboxyltransferase N-terminal domain (aa 28-290) (according to UniProt)
Structure
[PDB|2F9I] (from ''Staphylococcus aureus''; equivalent to [protein|E4997526CC534E38413030E90F7A135ECF65DF40|accD]-[protein|22B3039DF4D106F121DBC765F49878C9B324FDB2|accA], 64% identity) [Pubmed|16460018]
[AF|C0SP93]
Modification
phosphorylated on Arg-205 [Pubmed|22517742]
[wiki|Localization]
Membrane-proximal (Spotty) [Pubmed|16479537]
Expression and Regulation
Operons
genes
[gene|E4997526CC534E38413030E90F7A135ECF65DF40|accD]-[gene|22B3039DF4D106F121DBC765F49878C9B324FDB2|accA]
description
[Pubmed|22383849]
regulation
expressed under conditions that trigger sporulation ([wiki|Spo0A]) [Pubmed|14651647]
regulatory mechanism
[protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]: activation, [Pubmed|14651647], in [regulon|protein:2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A regulon]
Biological materials
Expression vectors
purification from ''B. subtilis'' with an C-terminal Strep-tag, for [wiki|SPINE], (in [wiki|pGP382]): pGP1724, available in [wiki|Jörg Stülke]'s lab
pGP3696: expression of [gene|search|accDA] by [wiki|pBQ200] in B. subtilis, available in [wiki|Jörg Stülke]'s lab
pGP1026, N-terminal Strep-tag, based on [wiki|pGP172], purification from ''E. coli'', available in [wiki|Jörg Stülke]'s lab
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
FLAG-tag construct
GP1497 (''mls'', based on [wiki|pGP1370]), available in [wiki|Jörg Stülke]'s lab
References
Reviews
Original Publications
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Time of last update: 2024-11-22 17:11:04
Author of last update: Dennis.wicke