atpA

atpA
168

ATP synthase, part of the F1 complex (subunit alpha)

locus
BSU_36830
Molecular weight
54.43 kDa
pI
5.04
Protein length
502 aaSequenceBlast
Gene length
1509 bpSequenceBlast
function
ATP synthesis
product
ATP synthase (subunit alpha)
essential
no
ec
3.6.3.14
synonyms
atpA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0056 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,783,878 3,785,386
The protein
Catalyzed reaction/ biological activity
ATP + 4 H+ + H2O --> ADP + 5 H+ + phosphate (according to UniProt) [http://www.youtube.com/watch?v=uOoHKCMAUMc see a video]
Protein family
ATPase alpha/beta chains family (with [protein|401459F105BC8A8342341D953AE259D6C3868AB6|fliI] and [protein|2A566C2CF8F7B86E05EEF4B7A268E8C102070025|atpD], according to UniProt)
Structure
[PDB|1SKY] ([protein|E3FADE979CE6AB6315F67812608FCE00CF4E2453|atpA]-[protein|2A566C2CF8F7B86E05EEF4B7A268E8C102070025|atpD] complex from ''Bacillus sp.'', 88% identity) [Pubmed|9261073]
[PDB|see here an overview on ATPase structure]
[AF|P37808]
Modification
phosphorylated on ser/ thr/ tyr [Pubmed|16493705]
Effectors of protein activity
ATPase activity is inhibited upon binding of Mg-ADP to [protein|2A566C2CF8F7B86E05EEF4B7A268E8C102070025|atpD] [pubmed|30580998]
[wiki|Localization]
membrane [Pubmed|18763711]
peripheral via theF0 complex
Additional information
belongs to the 100 most abundant proteins [PubMed|15378759]
Expression and Regulation
Operons
genes
[gene|8027AD5C3A92FB9B70E42C119FDEC697A64618C4|atpI]-[gene|CC1894D90AC17487A286B2909964916825055F93|atpB]-[gene|6CF7F6303425D8A51E1E01BF18823139E4259A8F|atpE]-[gene|87311FEA265AD3F5526C78D631E7E51BBF01F0D6|atpF]-[gene|E956A30D97B2941108880F3AD48620C4DB6E0EBD|atpH]-[gene|E3FADE979CE6AB6315F67812608FCE00CF4E2453|atpA]-[gene|492EEC8068043DA770F5DC1D54799C4366AE9A01|atpG]-[gene|2A566C2CF8F7B86E05EEF4B7A268E8C102070025|atpD]-[gene|A9DA64F8C7A60E8E2B1EDC99BEA1BD7DD0135AA6|atpC]
description
[Pubmed|9353933]
regulation
[protein|CEE73284EFC0DBE8870CE0B474922DED79475A57|rel] dependent downregulation (Class I) during stringent response [Pubmed|11948165]
the mRNA is processed between [gene|8027AD5C3A92FB9B70E42C119FDEC697A64618C4|atpI] and [gene|CC1894D90AC17487A286B2909964916825055F93|atpB] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|ricA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|ricF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT] complex [Pubmed|29794222]
regulatory mechanism
stringent response: negative regulation, in [regulon|other_regulator:stringent response|stringent response]
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[gene|8027AD5C3A92FB9B70E42C119FDEC697A64618C4|atpI]→[gene|A9DA64F8C7A60E8E2B1EDC99BEA1BD7DD0135AA6|atpC]

2024-12-01 15:21:39

Jstuelk

121

c30b9914547f38015d481b305686327f4f8f18d8

1C7DF7F5F812B06EFE55424A9900C106B30D346A

Biological materials
Mutant
BKE36830 ([gene|E3FADE979CE6AB6315F67812608FCE00CF4E2453|atpA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE36830 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GATGCTCACTTAGGTTTCAC, downstream forward: _UP4_TAACTCGAATGCTGATGAGA
BKK36830 ([gene|E3FADE979CE6AB6315F67812608FCE00CF4E2453|atpA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK36830 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GATGCTCACTTAGGTTTCAC, downstream forward: _UP4_TAACTCGAATGCTGATGAGA
References
Reviews
23356252,23341301,23267178,22822068,21524994,19489730,17208001,16730323
Research papers
30580998
Original Publications
36815589,7961438,15378759,16493705,18763711,25244289,9261073,26883633

E3FADE979CE6AB6315F67812608FCE00CF4E2453

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Time of last update: 2024-12-20 21:00:04

Author of last update: Jstuelk